摘要
目的 研究核转录因子-kB（NF—kB）在人类胰腺癌组织中的表达及与上皮细胞间质转化（EMT）标志物上皮性钙黏附蛋白（E—cad）和波形蛋白（Vimentin）的关系，探讨其与胰腺癌恶性生物学行为的关系。方法应用免疫组织化学法检测62例人类胰腺癌组织中NF—KB、上皮源性标志物E—cad蛋白、间质源性标志物Vimentin蛋白的表达，并分析它们相互间及其与临床病理因素间的关系。结果胰腺癌组织中NF—KB和Vimentin蛋白阳性表达率分别为81％（50／62）和61％（38／62），E—cad蛋白的表达缺失率为55％（34／62）。在胰腺癌组织中NF-kB的表达和Vimentin蛋白的阳性表达、E—cad蛋白的缺失表达均呈正相关（r值分别为0．343、0．352，均P〈0．05），并且NF—kB的阳性表达与胰腺癌的淋巴结转移（X2=11．761，P〈0．05）、远处转移（X2=9．225，P〈0．05）相关，而与患者性别、年龄、肿瘤部位、肿瘤类型及分化程度无关（P〉0．05）。E—cad蛋白的表达缺失、Vimentin蛋白的阳性表达与胰腺癌的淋巴结转移、远处转移均有相关性（X2值分别为6．914、4．984；7．753、5．144，均P〈0．05）。结论NF—KB在胰腺癌中表达增高，同时可能通过诱导胰腺癌组织的EMT而促进胰腺癌的浸润和转移。
Objective To investigate the expression of NF-KB and epithelial-mesenchymal transition （EMT） related markers E-cadherin and Vimentin proteins in human pancreatic cancer tissues and its relation with the malignant features. Methods The expression of NF-KB,E-cadherin and Vimentin proteins in 62 cases of pancreatic cancer tissues were detected by using immunohistochemistry and compared with the clinicopathological data of pancreatic cancer. Results The positive expression rate of NF-KB was 81% （50/ 62）, Vimentin protein increased of expression was 61% （38/62）, and E-cadherin protein loss of expression was 55 % （34/62） in pancreatic cancer. The positive expression rate of NF-KB was significantly related with the lymph node metastasis （X2=11.761, P 〈0.05）, distant metastasis （X2=9.225, P 〈0.05）, the absent expression of epithelial marker E-cadherin protein （r =0.352, P 〈0.05） and the positive expression of mesenehymal marker Vimentin protein （r =0.343, P 〈0.05）, but there was no relation with the patients gender, age, tumor location, tumor type and tumor differentiation （P 〉0.05）. tn addition, the significant correlation of E-cadherln expression loss and Vimentin expression with tumor lymph node metastasis and distant metastasis was found （X2 = 6.914, 4.984, 7.753, 5.144, P 〈0.05）. Conclusion The overexpression of NF-KB in pancreatic cancer may accelerate invasion and metastasis of pancreatic cancer through inducing EMT.
出处
《肿瘤研究与临床》
CAS
2011年第3期191-193,共3页
Cancer Research and Clinic
