摘要
目的研究大鼠股骨骨折合并脑外伤时血清leptin水平及骨痂组织中leptin的表达,探讨leptin与骨折合并脑外伤时骨折愈合加快的关系方法将64只SD大鼠随机分为四组:对照组、脑外伤组、骨折组和骨折合并脑外伤组各16只。建立大鼠中度脑损伤和骨折模型,分别于1、2、4、8﹑12周取血清,放射免疫法测血清中leptin水平,骨折标本摄X线片,骨痂组织作免疫组化。结果第2,4,8周与对照组相比骨折并脑外伤组、脑外伤组、骨折组大鼠血清leptin浓度均有显著性增高(P<0.05);第4周时骨折并脑外伤组大鼠血清leptin升高水平较其它两组明显(P<0.05);从第8周开始,手术3组大鼠血清leptin浓度都有所下降,但骨折并脑外伤组leptin浓度仍明显高于其它两组(P<0.05);第12周各组之间无统计学差异(P>0.05)。脑外伤合并骨折组形成骨痂体积在X线片上高于单纯股骨骨折组,差异有显著性(P<0.05)。骨折合并脑外伤组中骨痂组织leptin免疫组化染色平均阳性细胞数比骨折组有显著性增加(P<0.05)。结论伴有脑外伤的骨折愈合过程中,在一定时间内血清leptin水平升高,骨折愈合加快,提示Leptin是脑外伤后骨折愈合加速的因素之一。
Objective To study the relationship between leptin and enhanced osteogenesis after brain injury by investigating the expression of leptin in callus and the level of leptin in serum of rats with brain injury and femoral fracture.Methods 64 male SD rats were randomly divided into 4 groups：normal control,brain injury group,fracture group,fracture with brain injury group（n =16 per group）.Fracture and brain injury models were established.The rats were sacrificed 2,4,8and12weeks respectively after operation,and then the leptin concentration in serum was detected by rat leptin RIT kit and the callus was observed in the X-ray film and volume was measured.The callus was also examined with immunohistochemical staining.Results The serum levels of leptin in the brain injury group,fracture group or fracture with brain injury group significantly increased as compared with that in control group at the 2nd week after operation（P〈0.05）,The concentration of the leptin in serum of the fracture with brain injury group was higher than that of brain injury or fracture group 4,8weeks after operation（P〈0.05） and declined from the 8th week;at the 12th week after operation the difference in above four groups was not statistically significant.（P〈0.05）.The mean percentage of positive cells in callus of the fracture with brain injury group was higher than that of the fracture group（P〈0.05）.Callus formation of the fracture with brain injury group were larger and formed more quickly than those of the fracture group（P〈0.05）.Conclusions The femoral fracture recovers rapidly and leptin in the callus expresses highly in fracture with brain injury group,which suggests that leptin could yield the healing of the fracture.
出处
《齐齐哈尔医学院学报》
2011年第1期1-3,共3页
Journal of Qiqihar Medical University
