摘要
采用地高辛标记探针以分子杂交的方法对８５１例血清进行ＨＢＶＤＮＡ的检测，采用ＥＬＩＳＡ的方法对相同标本检测了抗－ＨＢｃＩｇＭ、ＨＢＶ二对半、抗－ＨＡＶＩｇＭ、抗－ＨＣＶ及抗－ＨＥＶＩｇＭ，发现ＨＢＶＤＮＡ阳性检出率与ＨＢｅＡｇ出现相关，且ＨＢｅＡｇ阴性的标本中仍有１０．２５％（４９／４７８）ＨＢＶＤＮＡ阳性，说明仅以ＨＢｅＡｇ出现与否判定是否存在ＨＢＶ的复制是不可靠的。同时，还发现ＨＢＶＤＮＡ的出现与肝炎类型无关。抗－ＨＢｃＩｇＭ阳性率则与ＨＢｅＡｇ的出现无关，而与肝炎类型相关。
To evaluat the value of hepatitis B virtus' DNA and anti-hepatitis B core antigen-IgM in clinical test,HBV-DNA in 851 specimen of serum was determined by molecule hybridizaion which was labled by digoxin.Hepatits B surface antigen,anti-hepatits B surface antigen antibody,Hepatits B e antigen,anti-hepatits B e antigen antibody,anti-hepatitis B core antigen antibody,anti-hepatits B core antigen-IgM,anti-hepatits A virtus-IgM,anti-hepatits C virtus -IgG and anti-hepatits E virtus-IgM were tested at same time.It showed that the positive rate of HBV-DNA han some relationship to the frequency of the occurrence of HBeAg.There were 10 25(49/478)of patients among the specimen of negative HBeAg whose HBV-DNA was positive.It suggested that it's not reliable to judge whether there was any duplication of HBV-DNA only by the appearance of HBeAg.Moreover,the HBV-DNA was not linked to the type of hepatitis B.The positive of anti-HBc-IgM had no relationship with HBeAg,but it was concerned in the type of Hepatitis B.
出处
《广东微量元素科学》
CAS
1999年第8期38-40,共3页
Trace Elements Science
