摘要
采用自制Ｔ载体，快速克隆ａｐｏＥ外显子４基因ＰＣＲ扩增产物，经双脱氧末端终止法测序证明为ε３等位基因。该方法快速、价廉，在基因诊断中便于推广。
To establish a rapid,simple and practical method for cloning polymerase chain reaction (PCR)products.Apolipoprotein E(apoE)exon 4 was amplified by using PCR.The PCR products was cloned into a plasmid Tvector.The recobinant pUCapoE is 3 allele used sequecing.Tvector cloning is considered a rapid,practical and inexpensive technique for cloning PCR products.
出处
《临床检验杂志》
CAS
CSCD
北大核心
1999年第4期206-208,共3页
Chinese Journal of Clinical Laboratory Science
