摘要
目的 检测卵巢癌 Ｙ Ｎ Ｚ２２ 位点有无杂合性丢失以及与临床病理的关系。方法 采用 Ｐ Ｃ Ｒ 技术、聚丙烯酰胺凝胶电泳银染法，对３０ 例卵巢癌及癌旁组织 Ｙ Ｎ Ｚ２２ 位点扩增片段长度多态性进行分析。结果 以癌旁组织提供的信息计算 Ｙ Ｎ Ｚ２２ 基因频率、杂合度，３０ 例卵巢癌中有信息个体 ２３ 例，杂合度 ７６．７％ ，其中 ３ 例观察到 Ｌ Ｏ Ｈ，频率为１３．０％ 。结论 卵巢癌组织中存在 Ｙ Ｎ Ｚ２２ 位点 Ｌ Ｏ Ｈ 现象，且与肿瘤组织分化程度及临床期别有关。
objective To detect the loss of heterozygosity(LOH) for YNZ22 loci in human ovarian carcinomas,and discuss the relationship between LOH and clinical pathology.Methods To analyse amplified fragment length polymorphism(Amp FLP) at locus YNZ22 of cancer and normal tissues in 30 cases with ovarian carcinomas by using PCR followed by PAGE and silver staining.Results The allele frequencies and heterozygosity were determined.Of 23 informative cases,the heterozygosity was 76.7%,the LOH was 13.0%.Conclusion The loss of heterozygosity for YNZ22 loci in ovarian carcinomas is related to the differentiation degrees and clinical stages.\;
出处
《实用肿瘤杂志》
CAS
北大核心
1999年第4期203-205,共3页
Journal of Practical Oncology
关键词
卵巢肿瘤
序列分析
杂合性丢失
YNZ22
ovarian neoplasms
polymerase chain reaction
sequential analysis
heterozygote
