摘要
目的 探索格林 巴利综合征 (GBS)的发病机制与空肠弯曲菌 (CJ)的关系以及我国GBS病前CJ感染率比较高的原因。方法 用改良的Skirrow方法在 1995～ 1997年收集的 4 7例GBS患者的粪便标本中分离CJ,以Penner方法进行血清学分型 ,对不能分离出CJ的患者粪便标本进行聚合酶链反应 (PCR) RFLP分型。对同期 171例CJ肠炎患者的CJ菌株用Penner方法进行血清学分型。结果 共分离出 6株CJ菌株 ,其中Penner 2型 3例、19型 1例、4型 1例、2 6型 1例。同期分离的 171例CJ肠炎患者的CJ菌株常见的血清型为Penner 2、19和 8型。PCR RFLP分型发现 3株DNA Ⅰ型 ,相当于Penner 19型。结论 我国引起GBS的CJ血清型和国外报告的相似 ,并且诱发GBS的血清型与CJ肠炎患者的血清型有极大相似性 ,这可能是GBS发病与CJ肠炎关系密切的原因。而Penner 2 6型也是引起GBS的血清型之一。
Objective To investigate the relationship between Campylobacter jejuni (CJ) serotype and the high incidence of Guillain Barre syndrome (GBS) following CJ enteritis in China. Methods CJ was isolated from fresh stool of 47 GBS patients in Beijing area from 1995 1997 with a combination of Skirrow method and micro filtration method. Serotpying of the CJ strains was performed with passive hemagglutination method with standard serum from CDC. 171 CJ strains from CJ enteritis in our hospital in the same period were serotyped with the same method. The stool from which no CJ could be isolated was typed with PCR RFLP method. Results 3 Penner 2, 1 Penner 4, 1 Penner 19 and 1 Penner 26 strains were isolated and serotyped. Three strains which could not be isolated were found by PCR RFLP method to be DNA I, which is equivalent to the type of Penner 19. There were some CJ strains which could be typed with serum from CDC. Conclusion CJ from GBS patients in Beijing area are similar to those reported in the world; it is suggested that CJ may cause GBS by molecular mimicry mechanism in China. The most common serotype of CJ from GBS was reported to be Penner 19 in Japan, while it was rare in CJ enteritis. However, the similarity of the serotypes between CJ from GBS patients and CJ from enteritis patients may explain the high incidence of GBS following CJ infection in China. Penner 26 is reported to be isolated from GBS patients for the first time.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1999年第7期455-457,共3页
Chinese Journal of Internal Medicine
基金
国家自然科学基金资助!(编号 :3 9470 2 5 9)
