摘要
本课题研究RA53 8、反义c ymc重组腺病毒对人胃癌 (SGC790 1 )、食管癌(EC1 0 9、EC871 2 )、正常人胚肺 2BS ( 2BS)及bcl 2高表达细胞系的体内外生物学作用及其分子机制。结果显示Ad RA53 8及Ad ASc myc对SGC790 1细胞体内外均具有明显的生长抑制及凋亡诱导作用 ,并能抑制其c myc、bcl 2、cyclinD1基因的表达及刺激bax基因的表达。对EC1 0 9、EC871 2、2BS及bcl 2基因高表达细胞系均无明显的生长抑制及凋亡诱导作用 ,对这些细胞系中c myc、bcl 2基因的表达均无调节作用。结果表明Ad RA53 8、Ad ASc myc对胃癌细胞具有显著的体内外生长抑制及凋亡诱导作用 ,其作用是c myc、bcl 2、bax、cyclinD1等一系列基因表达变化及其相互作用的结果。对食管癌、2BS及bcl 2高表达细胞系则无类似作用。
In this study, the biological effects and molecular mechanism of recombinant RA538 and antisense c myc adenovirus on human gastric ,esophageal, 2BS and high expression bcl 2 gene cancer cell lines were studied in vitro and in vivo. The results were as follows: Ad RA538 and Ad ASc myc could strongly inhibit cell growth and induce apoptosis of SGC7901 cells in vitro and in vivo, and could down regulate expression of c myc、bcl 2 and cyclinD1 gene, up regulate expression of bax gene. Ad RA538 or Ad AS c myc could not inhibit cell growth and induce apoptosis changes of EC109, EC8712, 2BS and high expression bcl 2 gene cancer cell lines, and could not down regulate expression of c myc and bcl 2 gene.The results indicated that: Ad RA538 or Ad AS c myc can inhibit growth and induce apoptosis of gastric cancer cell in vitro and in vivo. They relate to c myc, bcl 2, cyclinD1 and bax gene closely and play a key role on biologic effects in gastric cancer cells. Ad RA538 and Ad AS c myc could not produce relevant changes on esophageal cancer, 2BS and high expression bcl 2 gene cell lines.
出处
《生理科学进展》
CAS
CSCD
北大核心
1999年第3期227-230,共4页
Progress in Physiological Sciences
