摘要
[目的]研究1株米根霉菌体对胰酶粉浸提液中色素的生物吸附作用。[方法]分别考察菌体预处理、菌体添加量、菌球吸附粒径、溶液初始值、吸附时间、温度等对生物吸附脱色的影响。[结果]米根霉菌体对胰酶粉浸提液中色素的最佳脱色条件为:0.2 mol/L NaOH溶液热处理20 min,添加菌体量2%,菌体粒径0.6 mm,溶液初始pH值6.0,吸附脱色时间160 min、10℃,脱色率在89.5%以上,激肽原酶活性损耗小。[结论]米根霉菌体对胰酶粉浸提液脱色效果显著。
[Objective]To study decolorization of trypsin powder by Rhizopus oryzae biosorption.[Method] Fungus pretreatment,addition of fungus,pellets size,solution initial pH,adsorption time,temperature etc were investigated in decolorization of trypsin powder by Rhizopus oryzae biosorption.[Result]The optimal condition of decolorization of trypsin powder by Rhizopus oryzae biosorption was： heated 20 min by 0.2 mol/L NaOH,adding 2% fungus,0.6 mm pellets,solution initial pH6.0,adsorbed at 10 ℃ for 160 min.The decolorization rate was up 89.5%,and the losses of kallikrein activity was little.[Conclusion] Decolorization effect of trypsin powder by Rhizopus oryzae biosorption was remarkable.
出处
《安徽农业科学》
CAS
北大核心
2011年第9期5238-5240,共3页
Journal of Anhui Agricultural Sciences
基金
浙江省新苗人才计划项目(2010R428031)
关键词
米根霉菌
胰酶
生物吸附
脱色
Rhizopus oryzae
Trypsin powder
Biosorption
Decolorization
