摘要
提出了一种测定超滤膜切割分子量的简便方法，该法以鸡蛋清代替生化试剂作为标准分子量蛋白质，以卵清蛋白的截留率而不是截留率一分子量曲线作为判据来确定膜的切割分子量，切割分子量的确定范围为1万～6万．讨论了配制卵清蛋白溶液的适宜pH范围为10～11，实际使用质量分数为0．03％NaOH溶液即可；证明了用生化试剂和鸡蛋清配制的卵清蛋白溶液具有相同的分光光度性质，共享同一条浓度-吸光度工作曲线；阐明了比尔定律的适用范围，只需控制超滤前溶液的起始吸光度E。＝0．200左右就可用吸光度代替浓度计算膜对蛋白质的截留率；测定了不同鸡蛋中的卵清蛋白含量在10％～15％之间，同一只鸡蛋的蛋清中卵清蛋白的含量分布是相当均匀的；用已知切割分子量的膜测定了对卵清蛋白的截留率，并据此提出了确定切割分子量的判据；用细胞色素C的截留率证明了本法的适用性；还证明了用0．03％NaOH溶液配制的卵清蛋白溶液可在4～5℃下存放2～3周．
In this paper, a simple, convenient and very cheap method was presented to determine the molecularweight cut - off of the commonly used ultrafiltration membranes- The fresh egg white albumin is used as a stan-dard molecular weight protein instead of the biochemical agent egg white albumin, and the rejection ratio of theegg albumin is used as a determining criterion of the UF membrane molecular weight cut - off instead of the re-jection ratio - molecular weight curve. The range of the molecular weight cut - off determined is 10 000~60000dalton. This method is suitable to determine the noncharged UF membranes such as PS, PAN, PVD etc. Theshape of the membrane can be plane, tubular or hollow fiber. This method is also suitable for detemining themolecular weight cut - off of the membranes in the UF models.
出处
《膜科学与技术》
CAS
CSCD
1999年第5期44-50,共7页
Membrane Science and Technology
关键词
超滤膜
切割分子量
鸡蛋清
卵清蛋白
过滤
UF membrane molecular weight cut-off fresh egg white egg albumin
