摘要
目的 探讨碱性成纤维细胞生长因子（ｂ Ｆ Ｇ Ｆ）在卵巢上皮性肿瘤的生物学行为中的作用。方法 应用免疫组织化学技术对１０ 例正常卵巢组织和８０ 例卵巢上皮性肿瘤进行对照研究，同时应用 Ｍｉａｓ２０００ 图像分析系统进行定量分析，并研究恶性肿瘤中ｂ Ｆ Ｇ Ｆ的表达强度与临床分期以及肿瘤类型和病理分级间的关系。结果 ①ｂ Ｆ Ｇ Ｆ 主要在卵巢上皮性肿瘤细胞浆内表达，在交界型和恶性肿瘤中，部分阳性细胞同时存在细胞核表达。②ｂ Ｆ Ｇ Ｆ的表达强度随卵巢上皮性肿瘤的恶性程度增加而增强。正常卵巢组织ｂ Ｆ Ｇ Ｆ 密度为（３３５±３５２）％ ，良性上皮性肿瘤细胞为（１９２５±２１７３）％ ，交界型上皮性肿瘤组织细胞为（３３７８±１０８６）％ ，卵巢癌细胞为（４８１８±１２９３）％ 。提示ｂ Ｆ Ｇ Ｆ可能在卵巢癌的发生中起重要作用。③ｂ Ｆ Ｇ Ｆ的表达强度随 Ｆ Ｉ Ｇ Ｏ 分期的增高而增强。④ｂ Ｆ Ｇ Ｆ的表达强度随卵巢癌的分化程度降低而增强。⑤卵巢交界型浆液性囊腺瘤中ｂ Ｆ Ｇ Ｆ 的表达较交界型粘液性囊腺瘤明显增高，表明ｂ Ｆ Ｇ Ｆ在肿瘤恶变中可能起重要作用。结论ｂ Ｆ Ｇ Ｆ在卵巢肿瘤的发生、生长、浸润和转移等过程中起着重要作用 ?
Objective To determine the effect of basic fibroblast growth factor (bFGF) on the biological behaviour of ovarian epithelial neoplasm.Methods Ten cases of normal ovarian tissues and eighty cases of ovarian epithelial tumor tissues were detected by immunohistochemical methods. Mias 2000 Picture Analysis System was used to study the relationship of bFGF expression intensity and microvessel count、FIGO stage、pathological grade and classification of ovarian epithelial neoplasm.Results ①Expression of bFGF was mainly in cytoplasm and nucleus in several cells of borderline and malignant tumor.②The expression intensity of bFGF was closely related to the malignant degree of ovarian epithelial neoplasm. The density of bFGF expression was (3.35± 3.52)% in normal ovarian epithelium, (19.25±21.73)% in benign tumor, (33.78± 10.86) % in borderline tumor and (48.18±12.93)% in malignant tumor. The results indicated that bFGF might play an important role in carcinogenesis of ovarian epithelial neoplasm.③The expression intensity of bFGF was increased with the FIGO stage of ovarian tumor.④The expression intensity of bFGF was increased accompaning with the decrease of differentiation degree in ovian neoplasm.⑤In borderline tumor, expression intensity of bFGF in serous cystadenoma was significantly higher than in mucinous cystadenoma, which indicated bFGF might be an important factor in canceration of ovarian epithelial neoplasm.Conclusion bFGF may play important roles in carcinogenesis, development, invasion and metastasis of ovarian epithelial neoplasm.
出处
《中国修复重建外科杂志》
CAS
CSCD
1999年第5期299-304,共6页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金
关键词
卵巢肿瘤
上皮性肿瘤
BFGF
免疫组织化学
Basic fibroblast growth factor Ovarian epithelial neoplasm Expression Immunohistochemistry Foundation item: National Natural Science Foundation of China (39470721)
