摘要
目的:建立测定柏子仁霜中胡萝卜苷含量的方法,并比较不同产地柏子仁炮制后的霜品中胡萝卜苷的含量。方法:采用高效液相色谱-蒸发光散射检测法。色谱柱为Scienhomeinertex C18(150mm×4.6mm,5μm),流动相为甲醇-水(80∶20),流速为1.0mL·min-1,柱温为30℃;载气为氮气,气体压力为30.0psi,漂移管温度为80℃。结果:胡萝卜苷进样量在0.77～4.45μg范围内,其对数值与峰面积对数值呈良好线性关系(r=0.9997);平均回收率为96.55%,RSD=1.68%(n=6)。不同产地柏子仁霜中胡萝卜苷含量分别为0.4070%(河南辉县)、0.3281%(安徽淮北)、0.3384%(山东枣庄)、0.3261%(山东长清)。结论:本方法稳定、简便、快速,适用于柏子仁霜的质量控制。
OBJECTIVE：To establish the method for the content determination of daucosterol in Platycladus orientalis cream,and to compare the content of daucosterol in the cream of P.orientalis from different producing area after processed.METHODS：HPLC-ELSD method was adopted.Scienhome inertex C18（150 mm×4.6 mm,5 μm）was used with methanol-water（80：20） at flow rate of 1.0 mL·min-1.The column temperature was set at 30 ℃ and nitrogen was as carrier.The pressure of gas was 30.0 psi and the temperature of drift tube was 80 ℃.RESULTS：The linear range of daucosterol was 0.77-4.45 μg（r=0.999 7） with an average recovery of 96.55%（RSD=1.68%,n=6）.The contents of daucosterol in P.orientalis cream were 0.407 0%（from Huixian county of Henan province）,0.328 1%（from Huaibei of Anhui province）,0.338 4%（Zaozhuang of Shandong province）,0.326 1%（Changqing of Shandong province）,respectively.CONCLUSION：This method is stable,simple and rapid for the quality control of P.orientalis cream.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第15期1383-1384,共2页
China Pharmacy
基金
“十一五”国家科技支撑计划子课题(2006BAI09B06-05)
