摘要
目的:研究芦荟多糖软膏促浅Ⅱ度烫伤小鼠创面愈合的作用。方法:实验分为正常对照、芦荟多糖软膏、基质和京万红组,采用将脱毛区置于70℃恒温水浴中6 s的方法复制10%体表面积浅Ⅱ度烫伤模型,伤情经病理切片证实。各组创面分别用芦荟多糖软膏纱布(0.2 g.cm-2)、基质纱布(0.2 g.cm-2)、京万红纱布(0.2 g·cm-2)覆盖包扎固定后放回笼中饲养,每天换药1次,观察愈合时间,于伤后12 h和第1、3、5、7、9天,分别处死各组小鼠7只,取创面组织检测含水量、羟脯氨酸和一氧化氮(NO)含量,另取7只作为正常对照。结果:创面愈合时间芦荟多糖软膏组为(12.1±1.5)d,基质组为(15.2±3.2)d,京万红组为(12.5±1.9)d,芦荟多糖软膏组比基质组显著缩短(P<0.05)。伤后第1、3、5天,创面含水量芦荟多糖软膏组分别为(76.2±2.3)%、(65.7±3.1)%、(61.2±1.8)%,显著低于基质组(80.7±2.1)%、(70.8±3.1)%、(66.1±1.8)%,差异具有显著性(P<0.05)。伤后7～9 d各组均基本恢复到正常水平。伤后3、5、7、9 d芦荟多糖软膏组羟脯氨酸含量显著高于基质组和京万红组(P<0.05)。伤后12 h各组创面组织含水量达高峰,随后下降,伤后12 h、1 d,芦荟多糖软膏组和京万红组创面组织NO含量显著低于基质组(P<0.05)。结论:芦荟多糖软膏能有效减少烫伤后早期创面组织NO释放,减少渗出和水肿,增强创面胶原合成来促进创面愈合。
OBJECTIVE：To investigate the effects of Aloe polysaccharide ointment on wound healing of superficial second degree burn in rats.METHODS：Test divided into normal control group,aloe polysaccharide ointment group,matrix group and Jingwanhong group.Superficial second degree burn covering 10% of total body surface area was induced by immersing in 70 ℃ water for 6 second.The condition of burn was confirmed by pathological slice.The wounds were respectively covered with aloe polysaccharide ointment pledget（0.2 g·cm-2）,matrix pledget（0.2 g·cm-2）,and Jingwanhong pledget（0.2 g·cm-2）.The rats were caged separately,and the dressings were changed once a day.The rats wound healing time was observed in those groups.7 rats in each group were sacrificed and the biopsies of wound tissues were taken 12 hours and on 1st,3rd,5th,7th,9th day after burn for the content determination of water,hydroxyproline and NO.Another 7 rats were selected as control.RESULTS：The time of wound healing was（12.1±1.5） d in Aloe polysaccharide ointment group,（15.2±3.2） d in matrix group,and（12.5±1.9） d in Jingwanhong group respectively.The time for wound healing in Aloe polysaccharide ointment group was significantly shorter than that in matrix group（P0.05）.The water content in the wound tissue in Aloe polysaccharide ointment group on 1st,3rd,5th day after burn were（76.2±2.3）%,（65.7±3.1）%,（61.2±1.8）%,which was significantly lower than（80.7±2.1）%,（70.8±3.1）%,（66.1±1.8）% of matrix group（P0.05）.During 7-9 days after burn,all of them returned to the normal level.The content of hydroxyproline in the wound tissue in Aloe polysaccharide ointment group on 3rd,5th,7th,9th day after burn was higher than that in matrix group and Jingwanhong group（P0.05）.The content of water in wound tissue in each group reached the peak value 12 h after burn and then decreased.The content of NO in wound tissue in Aloe polysaccharide ointment group and Jingwanhong group were markedly lower than those in matrix group 12 h and 1 d after burn（P0.05）.CONCLUSION：Aloe polysaccharide ointment can accelerate wound healing by decreasing wound tissue NO release,lessening permeability and edema.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第15期1359-1361,共3页
China Pharmacy
基金
郧阳医学院研究生启动基金资助计划项目(2008QDJ1)
关键词
芦荟多糖软膏
浅Ⅱ度烫伤
一氧化氮
羟脯氨酸
Aloe polysaccharide ointment
Superficial second degree burn
NO
Hydroxyproline
