摘要
目的:观察四肢火器伤受不同剂量短波紫外线照射后,组织中羟脯氨酸含量的变化,以期分析紫外线照射对胶原蛋白合成的影响。方法:实验于2003-09/2004-09在解放军总医院理疗科实验室和解放军军事医学科学院放射医学研究所完成。选用新西兰大白兔90只,随机数字法分为3组:30mJ/cm2照射组、60mJ/cm2照射组和对照组,每组30只。麻醉动物后,以“五四”式手枪击兔右股部肌肉丰满处,造成贯通伤模型。常规清创后采用紫外线治疗仪分别对3组实验动物伤道进行30s(30mJ/cm2)照射,60s(60mJ/cm2)照射和无照射干预。在致伤后第2和3天继续在伤道内进行照射,日增加前次照射剂量的30%。采用化学法在伤后7,14,21,28,42,56和70d测量肉芽组织中羟脯氨酸含量的变化。结果:实验动物82只进入结果分析。①致伤后7d,各组大白兔组织中羟脯氨酸的含量无差别。②伤后14d,60mJ/cm2照射组羟脯氨酸的含量高于30mJ/cm2照射组和对照组[(11.91±0.49)mg/g,(10.88±0.61)mg/g,(10.35±0.85)mg/g,P<0.05];伤后21～56d,60mJ/cm2照射组伤口中的羟脯氨酸含量均高于30mJ/cm2照射组伤口和未照射组(P<0.01)。③伤后70d时,各组羟脯氨酸的含量下降接近正常组织水平,60mJ/cm2照射组仍稍高于30mJ/cm2照射组和未照射组[(10.02±0.65)mg/g,(9.85±0.54)mg/g,(9.27±0.43)mg/g,P<0.05]。④在伤后21～56d30mJ/cm2照射组伤口中羟脯氨酸的含量高于对照组(P<0.05)。结论:短波紫外线照射能促进伤口中羟脯氨酸的合成,增加胶原含量,60mJ/cm2照射的作用强于30mJ/cm2。
AIM： To investigate the changes of L-hydroxyproline （L-Hyp） level in the gunshot wounds on limbs after exposed to different doses of Ultraviolet C irradiation, so as to explore the effect of the radiation on collagen synthesis. METHODS： The experiment was performed in the laboratories of Department of Physiotherapy, General Hospital of Chinese PLA and Department of Pathology, Beijing Institute of Radiation Medicine from September 2003 to September 2004. Ninety New Zealand rabbits were randomly divided into 3 groups： 30 mJ/cm^2 irradiation group, 60 mJ/cm^2 irradiation group and control group with 30 rabbits in each group. The animal models of wound were established by shooting the muscle of right thigh with ＂54＂ type pistol. Immediately after debridement, the wounds of the two irradiation groups were irradiated under Ultraviolet for 30 seconds （30 mJ/cm^2） and 60seconds （60 mJ/cm^2）, respectively, the control group was not given irradiation. On days 2 and 3 after modeling, .the wounds were treated with intra-wound irradiation, with the dose increasing 30% gradationally. The changes of L-Hyp level in the granulation were detected with the chemistry method on days 7, 14, 21, 28, 42, 56 and 70. RESULTS： There were 82 animals involving in the result analysis. ①There was no difference in the L-Hyp level among the three groups at the 7^th day after modeling. ②The L-Hyp level of the 60 mJ/cm^2 group at the 14th day was significantly higher than both the 30 mJ/cm^2 group and the control group [（11.91±0.49） mg/g, （10.88±0.61） mg/g, （10.35±0.85） mg/g, P 〈 0.05]; from the 21^st to the 56th day, the L-Hyp level in the 60 mJ/cm^2 irradiation group was also significantly higher than both the 30 mJ/cm^2 group and the control group （P 〈 0.01）. ③At the 70^th day, the L-Hyp level of each group was decreased and approached to normal tissue level, but that of the 60 mJ/cm^2 group was still higher than both the 30 mJ/cm^2 group and the control group [（10.02±0.65） mg/g, （9.85±0.54） mg/g, （9.27±0.43） mg/g, P 〈 0.05]. ④The L-Hyp level of the 30 mJ/cm^2 group was higher than the control group from the 21^st to the 56^th day after modeling （P 〈 0.05）. CONCLUSION： Ultraviolet C irradiation is helpful to increase L-Hyp level in the gunshot wounds healing tissue, and accelerate the collagen synthesis. The effect of Ultraviolet C at dose of 60 mJ/cm^2 is superior to that of 30 mJ/cm^2.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第1期99-102,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
军队"十五"青年基金项目资助(01YQ17)~~
