摘要
采用DGGE-cloning测序技术与定量PCR技术相结合的方法,研究了转基因大豆对土壤中氨氧化细菌群落多样性的影响。定量PCR试验结果表明,相同的生长时期转基因大豆对氨氧化细菌数量没有显著的影响,而与此同时,土壤中的氨氧化细菌的数量呈现出随生长期先增加后减少的趋势;DGGE图谱分析表明,同一生长时期不同大豆土壤中的氨氧化细菌主要条带一致,这表明生长时期的影响明显大于转基因大豆对土壤氨氧化细菌的影响。
The genetically modified（GM）crops are more and more concerned by public;meanwhile more and more GM crops are planted in fields globally.Besides the food safety,the ecological risks are also gained focus.In order to deeply understand the effect of transgenic soybean on diversity of rhizoperic ammonia-oxidizing bacteria in soil,DGGE-cloning and quantitative PCR were used.Both the DGGE-cloning and qPCR results showed that seasonal changes were observed throughout the soybean growth stages,indicating the impact of crop growth stage overweigh that of exogenous gene insertion and transgenic soybean has not diversely affect on rhizosphere ammonia-oxidizing bacteria abundance and community composition in soil.
出处
《华北农学报》
CSCD
北大核心
2011年第1期210-214,共5页
Acta Agriculturae Boreali-Sinica
基金
中央级公益性科研院所基本科研业务费专项资金(2008-aepi-03)
