摘要
目的 研究阿司匹林（乙酰水杨酸，ＡＳＡ）对急性心肌梗死（ＡＭＩ）患者血小板与白细胞粘附的影响及其作用机制。方法 采用体外玫瑰花结形成实验检测血小板与白细胞粘附，观察ＡＳＡ 对两者粘附的作用；用１２５Ｉ标记的单克隆抗体测定血小板表面α颗粒膜蛋白１４０（ＧＭＰ１４０）的表达，观察ＡＳＡ对凝血酶诱导的血小板表面ＧＭＰ１４０ 表达的影响。结果 ＡＭＩ患者血小板与白细胞间粘附率增高，凝血酶通过诱导血小板表面ＧＭＰ１４０ 的表达促进血小板与白细胞粘附；阿司匹林（５００、５ ０００μｇ／ｍ ｌ）抑制ＡＭＩ患者血小板与白细胞间的粘附，抑制凝血酶诱导的正常人血小板与白细胞间的粘附以及凝血酶诱导的血小板表面ＧＭＰ１４０ 的表达。结论 ＡＳＡ一方面通过抑制ＧＭＰ１４０ 的表达，另一方面可能通过影响配体间的结合抑制血小板与白细胞之间的粘附，揭示了ＡＳＡ
Objective To investigate the effect and mechanism of aspirin(ASA) on platelet neutrophil adhesion Methods Using rosette forming assay to observe the effect of ASA on the binding of platelets to neutrophil and radioimmunoassay to observe the effect of ASA on the thrombin induced expression of GMP 140 on the surface of human platelets. Results Patients with acute myocardial infarction showed a significant increase in neutrophil platelet adhesion(63 3±7 8)%,( n=20,P <0 001)as compared with normal subjects(16 5±2 6)%( n =20).Thrombin promoted adhesion by increasing the expression of GMP 140 on the surface of human platelets.When the concentration of thrombin was 0 5 U/ml, the rate of neutrophil platelet adhesion and the number of molecular of GMP 140 on the surface of platelet was (64 2±4 2)%,and (1 80±0 20)×10 3 per platelet respectively. At high concentration,ASA significantly inhibited thrombin (0 5 U/ml)stimulated platelets binding to neutrophils and expressing GMP 140 on their surface. When the final concentration of ASA was 500,5000 μg/ml,the ratio of thrombin stimulated platelets binding neutrophils was (34 7±3 8)%,(21 2±3 6)% respectively ( n=20 ,P <0 01); the number of molecular of GMP 140 expressing on the surface of platelet was (1 02±0 24)×10 3,(0 68±0 18)×10 3 per platelet respectively (n=9, P<0 001 ) Conclusions It contributes to the antithrombosis of ASA that inhibiting platelet neutrophil adhesion by down expression of GMP 140 on the surface of human platelets. Key words It contributes to the antithrombosis of ASA that inhibiting platelet neutrophil adhesion by down expression of GMP 140 on the surface of human platelets. Key words Key words
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1999年第4期268-271,共4页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
