摘要
目的分析2007—2009年云南省腮腺炎病毒（MuV）分离株的SH和HN遗传特征。方法采用反转录一聚合酶链反应从Vero细胞分离的病毒株基因组中扩增出SH基因和HN基因，测序后用Mega4．1软件分析其遗传特征。结果云南省分离14株MuV的SH基凶其核苷酸和氨基酸同源性为98．3％～100．0％和96．5％～100．0％，与其他省份比较其同源性为92．6％～99．4％和87．7％～100．0％，其巾Wsh1和Wsh2与其他F基因型差异较大；与疫苗株同源性为84．5％～85．1％和77．2％；与其他基因型同源性为83．4％～90．9％和70．1％～86．0％。6株MuV分离株的HN基因与核苷酸和氨基酸同源性分别为99．3％～99．5％和99．1％～99．7％；与中国分离株SP株的核苷酸和氨基酸同源性均为99．8％；与其他基因型同源性为94．7％～96．8％和95．5％～99．1％；与疫苗株的同源性为92．4％～93．2％和95．5％～96．4％。结论2007-2009年云南省流行的MuV均为F基因型；其HN基凶比SH基因保守。
Objective To analyze genetic characterization of the small hydrophobic and hemagglutinin-neuraminidase genes of mumps virus （MuV） isolated in Yunnan province, China from 2007 to 2009. Methods Fourteen MuV strains were isolated in Yunnan, China from 2007 to 2009. Using RT-PCR, the SH gene fragments contained 316 nucleotides in all strains and HN gene of six strains were sequenced. The sequences were aligned with other mumps virus sequences downloaded from GenBank using Mega 4.1 software. Results Fourteen isolated strains were closely related to other reference strains of F genotypes. In SH gene, the homology of nucleotide and amino acid among the fourteen isolated strains were 98.3%-100.0% and 96.5%-100.0%, respectively, and 92.6%- 99.4% and 87.7%-100.0% of homology when compared with that of strains isolated from other provinces in China, respectively. Wshl and Wsh2 strains had less homology when compared to other strains of F genotypes. The fourteen strains had homology of 84.5%-85.1% and 77.2% compared to vaccine strains on nucleotide and amino acid, respectively, and had homology of 83.4%-90.9% and 70.1%-86.0% compared to that of other genotypes. In HN gene, the homology of nucleotide and amino acid among the six isolated strains were 99.3%-99.5% and 99.1%-99.7%, respectively, and also 99.8% and 99.8% of homology respectively when compared to the SP strain in China. All the six strains had homology of 92.4%-93.2% and 95.5%-96.4% when compared to the vaccine strains on nucleotide and amino acid, respectively, and had homology of 94.7%-96.8% and 95.5%-99.1% compared to other genotypes. Conclusion Fourteen strains isolated in Yunnan from 2007 to 2009 belonged to F genotype of MuV while the HN gene seemed more conservative than SH gene.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2011年第4期370-375,共6页
Chinese Journal of Epidemiology
基金
云南省基础研究面上项目（2008CD153）
