摘要
目的探讨养血生发胶囊对小鼠急性毒性的影响。方法采用经典的急性毒性试验方法,进行养血生发胶囊对小鼠的急性毒性研究。结果按生药量计算养血生发胶囊对小鼠的最大给药量(MLD)为24.0g.kg-1.d-1。相当于人日用剂量的420.0倍;给药后各小鼠急毒主要表现为安静、怠动,腹泻、毛色不华等毒性症状,给药后1天小鼠体重增长缓慢,与空白对照组有明显差异,给药后5天逐渐恢复,余未见明显异常,末次给药后2h血丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平明显增高,肝体比值增加。结论养血生发胶囊小鼠急性毒性实验虽未做出LD50和MTD,小鼠也无一死亡,但给药后小鼠有肝功能损伤、肝体比值增加,提示在超大剂量灌胃后具有一定的肝毒性,与临床报道相一致。
Objective To discuss the effect of Yangxue Shengfa capsules on mice＇s acute toxicity.Methods The classical method of acute toxicity were used to study on mice＇s caused by Yangxue Shengfa capsules.Results Caculated by Yangxue Shengfa capsules,the MLD is 24.0g·kg-1·d-1,which is equal to 420.0 times of 70kg people＇s daily dried medicinal herb expenses.The main acute toxicity symptoms of mouse are negligent action,diarrhea and hypokinesia.Compared with the control group,The mice＇s body-weight gainings were slow after 1 day＇s administration,which gradually normal after 5 day＇s administration and there were no other obvious abnormalities.The serum concentration of ALT and AST,the liver-body ratio all increased after 2 hours＇ administration.Conclusion The mice＇s acute toxicity of Yangxue Shengfa capsules could induce the liver injury although could not make the LD50 and MTD,and the mice did not appear dead.It demonstrated that the drug gavages certain dose to mice could cause obvious hepa-injury,which in accordance with the clinical ADR report.
出处
《中国药物警戒》
2011年第4期208-211,共4页
Chinese Journal of Pharmacovigilance
基金
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
山东省科技平台建设项目课题(2008GG2NS02021)
关键词
养血生发胶囊
小鼠
急性毒性
Yangxue Shengfa capsules
mice
acute toxicity
