摘要
对克隆在psb135质粒上的来自龙葵阿特拉津抗性生物型的psbA基因进行DNA序列分析,测得长为1384个核苷酸的全序列,包括该基因的全部编码区和5′上游顺序。该基因的核苷酸序列与另一个独立来源的龙葵阿特拉津抗性基因的核苷酸序列完全相同,在由核苷酸推导的氨基酸序列的基础上,比较了分别由龙葵抗阿特拉津和对阿特拉津敏感的psbA基因编码的32kD蛋白质的二级结构,并对其可能的含意进行了讨论。
The psbA gene cloned in pSB135 from Solatium nigrum atrazine-resistant biotype was se-quenced.It has the same nuclcotide sequence as the known atrazine resistance gene from another independent biotype of S.nigrum.On the basis of the deduced amino acid sequences the secondary structure of the 32kD proteins encoded by the pfbA genes were compared between atrazine-resistant and susceptible biotypes,and some indications from the protein structure comparison were discussed.
关键词
龙葵
叶绿体
核苷酸序列
阿特拉津
Solatium nigrum,Atrazine,Chloroplast,Nucleotide sequence
