摘要
目的研究大鼠脑出血不同时期Fas相关死亡域蛋白(FADD)表达和脑水肿及纳洛酮对其的影响,探讨纳洛酮脑保护机制。方法 126只SD雄性大鼠随机分为假手术组、脑出血组、纳洛酮治疗组,每组42只采用自体血脑内注射法建立脑出血动物模型,应用免疫组化方法检测脑出血后不同时间点血肿周边脑组中FADD表达情况,干湿比重法测脑水肿。结果出血后3hFADD和脑水肿升高,48～72h达到高峰,与脑出血组比较纳洛酮治疗组FADD和脑水肿表达显著减少(P<0.01)。结论脑出血后血肿周围细胞FADD表达和脑水肿明显增多,纳洛酮可能通过抑制FADD的表达,减轻脑水肿,对脑出血后脑组织有保护作用。
Objective To observe the protein expressions of Fas-associated with death domain protein（FADD） and brain edema in rats with intacerebral hemorrhage（ICH）,and to investigate the effects of naloxone on them in order to reveal the action mechanism of neuroprotection of naloxone.Methods 126 male SD rats were randomly divided into three groups：sham-operation group,intracerebral hemorrhage group and naloxone treatment group.The model of ICH was established in rats by intracerebral injection of autogenous blood.The protein expressions of FADD and brain edema in the tissues around the hemorrhagic region and the normal brain tissue were detected by immunohistochemistry.Results 3 hours after ICH,the protein expressions of FADD and brain edema were increased,which reached peak at 48h^72h,however,the expressions of FADD and brain edema in the region around hematoma in naloxone group was significantly decreased,as compared with those in model group.Conclusion The protein expressions of FADD and brain edema are obviously increased after ICH.The naloxone can protect the neurons by decreasing significantly the expressions of FADD and brain edema
出处
《河北医药》
CAS
2011年第6期810-812,共3页
Hebei Medical Journal
