摘要
目的调查一起外籍船员群体性腹泻的原因,及时采取相应措施防止腹泻疫情进一步扩散,并防止对环境可能造成的污染。方法结合流行病学调查结果,采集腹泻船员的肛拭子、粪便样本,处理后利用实时荧光PCR法对样本同时进行沙门氏菌、霍乱弧菌、副溶血性弧菌、大肠埃希氏菌O157:H7的检测;同时对以上样本进行增菌和分离培养;分离出的菌株进行生化鉴定和血清学分型。结果实时荧光PCR检测结果显示,14份样本(12份肛拭子、2份粪便)中13份沙门氏菌检测阳性,1份阴性,其他指标检测均为阴性;阳性样本中分离到的特征性菌落均符合沙门氏菌生化反应特征;血清学反应结果显示,以上菌株为沙门氏菌Ⅱ型。但从该外籍船上采集的食品、饮用水及环境样本中均未检出沙门氏菌。结论引起本次群体性腹泻事件的原因为沙门氏菌Ⅱ型感染。
Objective To collect the samples from infected sailors and develop a inspection at laboratory for the pathogeny of the group diarrhea event.To take some measures to protect the healthy sailors of the infected foreign ship and to control the spread of the epidemic.Method Considering epidemiological analysis,the rectal swabs,stools were collected and the pathogens as Salmonella,Vibrio cholera,Vibrio parahemolyticus and Escherichia coli O157：H7 were detected with real-time fluorescent PCR.The methods of biochemical analysis and serological typing were also used to identify the separated strains.Result The result of real-time fluorescent PCR showed that 13 samples were positive of Salmonella nucleic acid and there were not any other pathogens were detected.The positive separated strains were identified to be Salmonella II with biochemical and serological analysis.Conclusion The pathogeny of the group diarrhea event is the infection of Salmonella II Foods and drinking water are presumbed to be the most possible pollutant source,but we have not found the pathogen of Salmonella from either all kinds of foods,drinks or environmental samples.
出处
《中国国境卫生检疫杂志》
CAS
2011年第1期19-20,51,共3页
Chinese Journal of Frontier Health and Quarantine
关键词
外轮
群体性
腹泻
检测
沙门氏菌
Foreign ship
Group event
Diarrhea
detection
Salmonella
