摘要
目的探讨改进SC.SS培养基检测沙门氏菌中应用价值。方法8 229例标本来自疫区伤寒患者、健康体检肛拭子采集。经增菌后分离鉴定。并将作40份标本在同一型沙门氏菌进行增菌、分离鉴定。并将结果进行比较分析。结果1999～2004年检出率分别为0.13%、0.17%、0.33%、1.15%、1.83%、2.40%。原总阳性率为0.20%,改后总阳性率为1.89%。经卡方检验(χ2=52.78,P<0.01)差别有高度显著性。结论改后和原SC.SS相比,检测阳性率明显提高,且平板上菌落,硫化氢明显增多。平板颜色鲜艳,保留时间又长,省时又省力。为基层检测工作提供新方法以及厂家生产培养基提供新的配方。
Objective To explore value of improved SC.SS culture medium for detection of Salmonella.Methods 8 229 cases of specimen of anal swab from typhoid patients and normal person in infected areas,were enriched and then isolated for detection.40 samples of them were detected for isolation and identification of same type pf salmonella bacteria.the results were comparated.Results During 1999-2004 annual rates of 0.13%,0.17%,0.33%,1.15%,1.83%,2.40% respectively.The original total positive rate was 0.20 percent,to overall positive rate was 1.89% showing significant differences by chi-square test（χ2=52.78P0.01）.Conclusion The comparison showed that the positive rates,plate colony and hydrogen sulfide significantly increased using of the improved SC.SS culture medium with bright colors in the flat,and long retention time,a time-saving comparing to the original medium.The new method for grass-roots units and new formula were provided for Salmonella detection.
出处
《中国公共卫生管理》
2010年第2期149-150,共2页
Chinese Journal of Public Health Management
