摘要
为观察急性心肌梗死（ＡＭＩ）患者血小板活化状态的改变及临床意义，采用血小板表面抗人活化型α颗粒膜蛋白－１４０（ＧＭＰ－１４０）及血小板膜糖蛋白Ⅱｂ·Ⅲａ复合物（ＧＰⅡｂ·Ⅲａ）的单克隆抗体，以酶联免疫法检测１９例ＡＭＩ患者血小板活化状态，将其与２５例正常对照者对比，并分别与心肌酶及血栓素（ＴＸＢ_２）作相关分析。结果ＡＭＩ组血小板ＧＭＰ－１４０、ＧＰⅡｂ·Ⅲａ分子数明显高于正常对照组（均Ｐ＜０．０１）。同时显示ＧＭＰ－１４０和ＧＰⅡｂ·Ⅲａ增高均与血清肌酸激酶、肌酸激酶同功酶、乳酸脱氢酶、ＴＸＢ_２呈正相关（均Ｐ＜０．０１）。表明血小板活化功能检测对ＡＭＩ的诊治有一定临床价值。
In order to study the platelet activation in patients with acute myocardial infarction(AMI)and unstable angina and its clinical significance. Methods: The monocolnal antibodies of a-granule membrane protein-140 (GMP-140 ) and platelets glycoprotein Ⅱb·Ⅲa complex(GPⅡb· Ⅲa)were used resectively to evaluate the platelet activation in 19 cases with AMI and 25 normal controls by using enzyme-linked immunoassays. The correlation between GMP-140,GP Ⅱb·Ⅲa, myocardial enzymes and TXB_2 were analysed in the meantime. Results: The results showed that the number of of GMP-140 molecules on platelet surface in patients at the acute stage of AMI was significantly increased as versus normal controls(P< 0 .05). The levels of GMP-140 interrelated significantly with CK, CK-MB, LDH, TXB_2 (all P < 0 .01 ). The number of GP Ⅱb·Ⅲa complex molecules on platelet membrane surface in AMI cases were significantly higher than normal subjects(P < 0 .05 ). The data showed good correlations between GP Ⅱb·Ⅲa and CK, CK-MB, LDH, TXB_2 (all P< 0. 01) . Conclusion: The de- tection of platelet activation had certain clinical value in diagnosing acute myocardial infarction.
出处
《天津医药》
CAS
1999年第11期658-659,共2页
Tianjin Medical Journal
关键词
膜蛋白质
血小板
膜糖蛋白
心肌梗塞
AMI
membrane protein platelets membrane glycoproteins myocardial infarction acute disease
