摘要
目的 观察短波紫外线（ＵＶＣ） 照射正常人外周血单个核细胞凋亡及白细胞介素２（ＩＬ－ ２） 、ＩＬ－ １２ 水平的影响。方法 采用５０ ｍＪ／ｃｍ２ ，２００ ｍＪ／ｃｍ２ 和４００ ｍＪ／ｃｍ２ 不同剂量的ＵＶＣ照射人体外周血单个核细胞，培养２４ 小时后用双抗体夹心ＥＬＩＳＡ 法检测细胞凋亡及ＩＬ－ ２ 、ＩＬ－ １２的含量。结果 ２００ ｍＪ／ｃｍ２ 、４００ ｍＪ／ｃｍ２ＵＶＣ 照射可明显诱发细胞凋亡（ｐｇ／ｍｌ） ，分别为４５ ．６８ ±１．３５，４６ ．６２ ±１．０２；照射后ＩＬ－ ２ 含量（ｐｇ／ｍｌ）明显下降（分别为１７６．８８±６ ．９７ ，１７４．１０ ±５ ．３６），与对照组（ｓＡＰＯ－ １／Ｆａｓ ４１．０９ ±１ ．６９ ，ＩＬ－ ２ 为１９２．９８ ±１１．９７ ｐｇ／ｍｌ） 比较差异均有非常显著性（ Ｐ＜０．０１） ，而ＩＬ－ １２ 含量无明显变化。结论 ＵＶＣ 照射可诱发人外周血单个核细胞凋亡，此外凋亡可能是ＵＶＣ引起的ＩＬ－ ２ 缺失所致。
Objective To observe the influence of ultraviolet C(UVC) irradiation on apoptosis of human peripheral blood monocytes and interleukin-2(IL-2),interleukin-12(IL-12).Methods The human peripheral blood monocytes in vitro were irradiated with different UVC doses(50 mJ/cm 2, 200 mJ/cm 2, 400 mJ/cm 2).After culture of 24 hours,the monocyte apoptosis and IL-2,IL-12 level were determined by double-antibody sandwich ELISA method.Results Using 200 mJ/cm 2 and 400 mJ/cm 2 of UVC irradiation,the monocyte apoptosis was obviously induced,it was 45.68±1.35 pg/ml and 46.62± 1.02 pg/ml respectively ;the IL-2 level was markedly reduced(that is,176.88±6.97 pg/ml and 174.10± 5.36 pg/ml separately).Compared with the control group(sAPO-1/Fas 42.09±1.69 pg/ml and IL-2 192.98±11.97 pg/ml),there was a significant difference(P<0.01).At the same time,the IL-12 level hadn't changed obviously.Conclusion It is showed that the UVC irradiation may cause the apoptosis of human peripheral blood monocytes.In addition,the apoptosis is probably resulting from the IL-2 lack after ultraviolet C irradiation.
出处
《中华理疗杂志》
1999年第5期278-279,共2页
Chinese Journal of Physical Therapy
