摘要
目的:建立同时测定艾迪注射液中5种苷类成分的RP-HPLC-ELSD方法。方法:Kromasil C18色谱柱(4.6 mm×250 mm,5.0μm);流动相乙腈-水,梯度洗脱;流速1 mL.min-1;柱温35℃。SHIMADZU ELSD-LTⅡ型检测器;检测温度40℃;载气高纯氮气;载气压力0.35 MPa。结果:紫丁香苷在21.2～212 mg·L-1,人参皂苷Rg1在30.8～308 mg·L-1,人参皂苷Re在24.8～248 mg·L-1,人参皂苷Rb1在25.6～256 mg·L-1,黄芪甲苷在31.2～312 mg·L-1,峰面积A的对数值lgA与相应质量M(μg)的对数值lgM呈良好的线性关系(n=6)。5种成分的平均回收率(n=9)均高于97.6%,RSD均小于1.1%。结论:该方法简便、准确,重复性好,为艾迪注射液的质量控制提供了方法。
Objective： To develop a RP-HPLC-ELSD method for the simultaneous determination of five glycosides in Aidi injection. Method： The separation was carried out at 35 ℃ on a Kromasil Cl8 column （4. 6 mm×250 mm, 5μm ） eluted with acetonitrile and water as the mobile phases in gradient mode. The flow rate was 1.0 mL · min ^-1. The SHIMADZU ELSD-LT Ⅱ detector was used. The detector temperature was at 40 ℃ and the pressure of carrier gas N2 was 0. 35 MPa. Result： The linear response （ the natural logarithm of peak areas with corresponding mass ） ranges were 21.2-212 mg ·L^-1 for syringin, 30. 8-308 mg· L^-1 for ginsenoside Rg1 , 24. 8-248 mg · L-1 for ginsenoside Re, 25.6-256 mg· L^-1 for ginsenoside Rbl, 31.2-312 mg · L^-1, for astragaloside IV, respectively. The average recoveries （n = 9） of five glycosides were greater than 97.6%, and RSD were less than 1.1%. Conclusion： The results demonstrated that this method had adequate accuracy and selectivity to measure the concentrations of five glycosides in Aidi injection.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第6期706-708,共3页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX09308-003
2011ZX09201-201-16)
