摘要
通过构建皱纹盘鲍(Haliotis discus Hannai)cDNA文库并进行大规模测序,获得了1282条高质量的ESTs序列,对这些序列进行拼接,获得了包括650条序列在内的244组片段重叠群(Contigs)和632个单一序列(Singletons)。使用BlastX软件对876个Unigenes(Unigene为Contig和Singleton的总和)进行同源性对比,有393个获得了功能提示,占总基因数的45%。根据EST信息,采用RACE方法,首次得到皱纹盘鲍神经肽Y(neuropeptide Y,NPY)受体基因,并对其结构进行了分析。结果表明,该受体基因cDNA由1534个核苷酸组成,编码区1050bp,可以编码350个氨基酸;该多肽的分子量为39.56kda,等电点为9.57,具有7个明显的跨膜螺旋且TM7区有NPXXY模体。
A cDNA library was constructed from liver and kidney of abalone（Haliotis discus Hannai） and 1445 clones were selected for sequencing.One thousand and two hundred eighty two high qualitative ESTs were obtained through bioinformatics analyses.Splicing of these sequences,244 contigs（consisting of 650 sequences） and 632 singletons were obtained.Using BlastX program for the Homologous comparison,393 unigenes shared significant homology with known sequences in protein database of NCBI accounted for 45% of the number of unigenes.Among that,63（16%） were immune-related.The full length of the NPY（neuropeptide Y） receptor cDNA was cloned through the 5＇-RACE with the specific primers based on the sequence of the EST.The full length of NPY receptor cDNA from Haliotis discus hannai was 1534bp and encoded 350 amino acid.BLAST analysis revealed that the NPY receptor gene from Haliotis discus hannai shared high identity with the NPY receptor genes from other organisms in amino acid.This NPY receptor have a predicted molecular of 39.56 kDa and theoretical isoelectric point of 9.57,receptively.
出处
《海洋湖沼通报》
CSCD
北大核心
2011年第1期94-100,共7页
Transactions of Oceanology and Limnology
基金
基于生态系统的典型海域生物资源综合修复与调控技术研究及示范(200905019)
海洋一所基本科研业务专项(GY02-2008G45)
