摘要
目的:探讨心脏κ阿片受体对心肌Cx43基因和其蛋白表达的调节作用。方法:将35只成年雄性SD大鼠随机进行实验分组。大鼠麻醉后,经股静脉分别给于κ阿片受体和β肾上腺素受体的激动剂或阻断剂。30 min后,剪取左心室心肌组织,用RT-PCR技术检测大鼠心肌组织中Cx43 mRNA的表达水平。用免疫组化染色法检测Cx43总蛋白和磷酸化的Cx43蛋白(P-Cx43)的表达。结果:κ阿片受体激动剂U50,488H可使Cx43 mRNA的表达明显降低;而β肾上腺素受体激动剂异丙肾上腺素可明显提高Cx43 mRNA的表达(P<0.05)。β肾上腺素受体阻断剂普萘洛尔和κ阿片受体激动剂U50,488H均可明显抑制异丙肾上腺素的上述作用。异丙肾上腺素可使P-Cx43的表达降低;而普萘洛尔和U50,488H则可抑制异丙肾上腺素所致P-Cx43表达的降低。上述U50,488H的作用均可被κ阿片受体选择性阻断剂Nor-BNI所阻断。结论:κ阿片受体可通过影响β肾上腺素受体进而调节Cx43的功能。
AIM： To explore the effects and mechanism of κ opioid receptor and β adrenergic receptor stimulation on the expression of Cx43 gene and its protein in myocardium.METHODS： 40 male SD rats（250±20） g were randomly divided into different groups.Animals were given different agents according to experimental condition including κ opioid receptor and β adrenergic receptor agonist and antagonist.After 30 min,the left ventricular tissue was adopted for the later determination.RT-PCR was used to detect the amount of Cx43 mRNA and an immunohistochemical staining was used to detect the expression of Cx43 protein in myocardial cells.RESULTS： Compared with the control,the expression of Cx43 mRNA decreased with administration of U50,488H（a selective κ opioid receptor agonist）,while increased with administration of isoproterenol,a β adrenergic receptor agonist.Meanwhile,U50,488H and propranolol,a non-specific β adrenergic receptor antagonist,inhibited the increased expression of Cx43 mRNA induced by isoproterenol（P〈0.05）.Accordingly,compared with control,the phosphorylated Cx43 protein was decreased with administration of isopreterenol.Meanwhile,both U50,488H and propranolol inhibited the decreased expression of phosphorylated Cx43 induced by isoproterenol.CONCLUSION： κ opioid receptor agonist U50,488H affects cardiac Cx43 expression via inhibiting β-adrenergic receptor signaling pathway.
出处
《心脏杂志》
CAS
2011年第1期35-38,共4页
Chinese Heart Journal
基金
国家自然科学基金项目资助(30770802
30971060)
