摘要
从化学、仿生物、细胞三个层次，水溶、脂溶两种体系研究了脂溶性茶多酚（ＬＴＰ）的抗氧化特性，并与维生素Ｅ（ＶＥ ），绿茶多酚（ＧＴＰ）等进行了比较⒚结果表明：对水溶性体系中的·ＯＨ，巨噬细胞呼吸爆发产生的氧自由基的清除效果为ＧＴＰ＞ ＬＴＰ＞ ＶＥ⒚对大分子稳定的二苯基苦基肼基（ＤＰＰＨ）自由基，ＬＴＰ，ＧＴＰ效果接近且均显著优于ＶＥ⒚在膜体系中，ＧＴＰ对Ｆｅ２＋ 诱导的膜脂质过氧化的抑制作用优于ＬＴＰ，ＶＥ⒚而ＶＥ 对２，２＇偶氮２，４二甲基戊腈（ＡＭＶＮ）诱导的膜脂质过氧化的抑制作用显著优于ＬＴＰ， ＧＴＰ，当浓度大于５０ μｇ／ｍ Ｌ时，ＬＴＰ的抑制作用保持在２５％ 左右并显著优于ＧＴＰ⒚ＬＴＰ基本保留了原有茶多酚优良的抗氧化特性，在脂溶性体系中效果较好⒚同时给出了抗氧化剂在各体系中 ５０％抑制率或清除率时的浓度，即ＩＣ５０
In order to investigate the antioxidant properties of the novel antioxidant lipophilic tea polyphenols (LTP),we established three levels of models from chemical, imitate biological and biological systems, and compared their activities with Vitamin E (V E) and green tea polyphenols (GTP).It was found that their ability to scavenge hydroxyl radicals (·OH), and reactive oxygen species generated by macrophage's breathe burst in biological system decreased in the order of GTP>LTP>V E. In big molecular stable free radical DPPH (1,1 diphenyl 2 picylhydrazyl) system, GTP and LTP have close scavenge activities and significantly better than that of V E. It was also found that they could inhibit lipid peroxidation of liposomal suspension induced by iron and was classified as follows: GTP>V E≈LTP. The inhibitary activity on the lipid peroxidation initiated by 2,2' azobis(2,4 dimethylvaleronitrile)(AMVN) is decreased in order of V E>LTP≈GTP,when the concentration is more than 50 μg/mL ,their antioxidant activities could be classified as follows: V E >LTP>GTP. The concentration of these compounds inhibiting 50% lipid peroxidation (IC 50 )or scavenging 50% free radicals(SC 50 ) were also given in the paper.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
1999年第5期529-534,共6页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省教委资助
关键词
脂溶性茶多酚
维生素E
绿茶多酚
抗氧化特性
lipophilic tea polyphenols
Vitamin E
green tea polyphenols
antioxidant properties
