摘要
[目的]寻求诱导也门铁叶片愈伤组织的最佳激素配方。[方法]以也门铁无菌苗叶片为外植体,以MS为基本培养基,分别添加不同浓度的2,4-D、BA和NAA,配制成24种激素组合,通过离体组织培养,探讨不同激素配比对也门铁叶片愈伤组织的诱导效果。[结果]当NAA为0 mg/L,2,4-D与BA 2种激素以不同浓度组合,在2,4-D浓度是BA浓度的1.0~1.5倍时,也门铁愈伤组织诱导效率较高,但质量较差。当2,4-D为0 mg/L时,BA与NAA 2种激素组合的诱导率为0。当2,4-D、NAA与BA 3种激素组合且2,4-D与NAA的浓度比值为1时,也门铁愈伤组织的诱导时间短,愈伤组织质量较好。也门铁叶片愈伤组织诱导的最佳培养基配方是MS＋0.5 mg/L2,4-D＋0.5 mg/L NAA＋1.0 mg/L BA,诱导率为44.4%。[结论]该研究为探索高效的也门铁组培快繁新方法提供科学依据。
[Objective] The study aimed to seek for the optimum hormone formula for inducing Dracaena fragrans ker-Gawl leaf callus.[Method] With the leaves of Dracaena fragrans Ker-Gawl aseptic seedlings as the explants and MS medium as the basic medium,the 2,4-D,NAA and BA with different concn.were added into the basic medium to make into 24 kinds of hormone combinations and the effects of the hormones with different matching rates on callus induction of D.fragrans.were discuss through the tissue culture in vitro.[Result] When NAA was 0 mg/L,in 2 kinds of hormone combinations including 2,4-D and BA with the concn.of 2,4-D being 1.0-1.5 times of BA,the callus induction of D.fragrans was higher,but the callus quality was poor.When 2,4-D was 0 mg/L,the callus induction of 2 kinds of hormone combinations including BA and NAA was 0.In 3 kinds of hormone combinations including 2,4-D,BA and NAA with the concn.ratio of 2,4-D and NAA being 1,the callus of D.fragrans was short in induction time and better in the quality.The optimum hormone formula for induction of D.fragrans callus was MS＋0.5 mg/L 2,4-D＋0.5 mg/L NAA＋1.0 mg/L BA,with the induction rate of 44.4%.[Conclusion] The study provided the scientific basis for exploring the new and efficient method of tissue culture and rapid propagation of D.fragrans.
出处
《安徽农业科学》
CAS
北大核心
2011年第5期2546-2547,共2页
Journal of Anhui Agricultural Sciences
关键词
也门铁
愈伤诱导
组织培养
Dracaena fragrans Ker-Gawl
Callus Induction
Tissue culture
