摘要
目的探讨乌司他丁(Ulinastatin for injection,UTI)和泰索帝(Taxotere,TXT)对人乳腺癌细胞MDA-MB-231(ER-)增殖、侵袭及血管内皮生长因子-C(Vascular endothelial growth factor-C,VEGF-C)、碱性成纤维细胞生长因子(Basic fibroblastgrowth factor,bFGF)、神经生长因子(Nerve growth factor,NGF)mRNA转录水平的影响。方法体外培养MDA-MB-231细胞,随机分为4组:对照组(只加RPMI1640培养液)、UTI组(终浓度800 U/ml)、TXT组(3.7μg/ml)、UTI+TXT组(800 U/ml UTI+3.7μg/ml TXT)。给药后24、48、72 h,采用MTT法检测细胞增殖水平;24 h,采用Transwell侵袭小室法检测细胞侵袭能力,实时荧光定量PCR法检测细胞VEGF-C、bFGF、NGF基因mRNA的转录水平。结果 UTI、TXT及UTI+TXT呈时间依赖性抑制MDA-MB-231细胞的增殖(P均<0.05),并显著抑制MDA-MB-231细胞的侵袭能力(P均<0.01)及VEGF-C、bFGF、NGF基因mRNA的转录水平(P均<0.05);TXT组与UTI+TXT组NGF基因mRNA转录水平比较,差异无统计学意义(P>0.05);对细胞增殖、侵袭及VEGF-C、bFGF、NGF基因mRNA转录的抑制作用UTI+TXT>TXT>UTI。结论 UTI和TXT均可显著抑制乳腺癌细胞MDA-MB-231增殖、侵袭,UTI可增强TXT的抑制作用,其机制可能与下调VEGF-C、bFGF、NGF mRNA的表达有关。
Objective To investigate the effects of ulinastatin for injection（UTI）,taxotere（TXT） on the proliferation and invasion of breast cancer MDA-MB-231（ER-） cells as well as the transcription levels of vascular endothelial growth factor-C（VEGF-C）,basic fibroblast growth factor（bFGF） and nerve growth factor（NGF） mRNAs.Methods The MDA-MB-231 cells cultured in vitro were randomly divided into four groups,i.e.control（RPMI1640 medium）,UTI（to a final concentration of 800 U / ml）,TXT（3.7 μg / ml） and UTI ＋ TXT（800 U / ml UIT ＋ 3.7 μg / ml TXT） groups,then determined for proliferation level by MTT method 24,48 and 72 h,for invasion ability by Transwell test 24 h,and for transcription levels of VEGF-C,bFGF and NGF mRNAs by fluorescent real-time quantitative PCR 24 h after treatment.Results UTI,TXT and UIT ＋ TXT showed time-dependent inhibitory effect on the proliferation of MDA-MB-231 cells（P 0.05）,and significantly inhibited the cell invasion（P 0.01） as well as the transcriptions of VEGF-C,bFGF and NGF mRNAs（P 0.05）.The transcription levels of NGF mRNA in TXT and UTI ＋ TXT groups showed no significant difference（P 0.05）.In the turn of inhibitory effects on cell proliferation and invasion as well as transcription of VEGF-C,bFGF and NGF mRNAs,the groups were UTI ＋ TXT,TXT and UTI.Conclusion UTI and TXT inhibited the proliferation and invasion of MDA-MB-231 cells significantly,and UTI enhanced the inhibitory effect of TXT,by a potential mechanism of down-regulating the expressions of VEGF-C,bFGF and NGF mRNAs.
出处
《中国生物制品学杂志》
CAS
CSCD
2011年第2期203-207,共5页
Chinese Journal of Biologicals
基金
重庆市科学技术委员会科技攻关资助项目(CSCT
2008AC5082)
关键词
乌司他丁
泰索帝
乳腺肿瘤
细胞增殖
肿瘤侵润
药理作用分子作用机制
Ulinastatin for injection（UTI）
Taxotere（TXT）
Breast tumor
Cell proliferation
Tumor invasion
Molecular mechanism of the pharmacological action
