摘要
应用体外器官培养研究人鼻咽癌不同克隆细胞株的侵袭特性郁多男，高进（中国医学科学院中国协和医科大学北京基础医学研究所，北京１００００５）研究肿瘤细胞体外侵袭能力的方法很多，如利用单层内皮细胞［１］、人类羊膜［２，３］、小鼠膀胱［４］、眼晶状体囊［５］以...
Abstract Using limiting dilution assay,variants were cloned from the CNE-2Z cell line.The variants CNE2L2,CNE2H2,CNE2L4 and CNE2M2,with cell electrophoresis rates which differed greatly,were assayed for their invasive capahilities by observing their ability to invade preculture embryonic chicken heart fragment(PHF)in organ culture using a gyrotory shaker system.The results indicated that CNE2L2 variant cells invaded 9-day-old chicken embryonic heart fragments at a rate significantly higher than those seen using CNE2L4 and CNE2M2 in 7-day assay. Computer image analysis showed that the CNE2L2 cells occupied 80.2%of the PHF area much greater than that occupied by CNE2L4(46.5%)or CNE2M2(36.5%).This experiment also showed that the electrophoresis rate of the CNE2Z variants was positively related to their invasive capabilities in vitro.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1994年第4期317-322,共6页
Acta Academiae Medicinae Sinicae
