摘要
为了将AFLP技术应用到企鹅珍珠贝相关研究中,以企鹅珍珠贝为材料,采用SDS-CTAB改进法DNA提取高质量的基因组DNA,通过对AFLP双酶切体系?连接体系?预扩增体系及选择性扩增体系中关键因素进行优化,建立了适用于企鹅珍珠贝的AFLP反应体系。同时以3个企鹅珍珠贝地理群体为材料,采用新建立AFLP反应体系从256对引物组合中筛选出10对多态性高的AFLP引物组合,说明建立的优化体系可用于企鹅珍珠贝的AFLP分析。
To establish an efficient and stable amplified fragment length polymorphism（AFLP） analysis system for Pteria penguin,SDS-CTAB modified method was used to extract high-quality genomic DNA.The key factors were explored and optimized for enzyme-digested reaction,ligation,pre-amplification and selective amplification.10 pairs of polymorphism primers were screened from 256 pairs of primers when new AFLP analysis system was used to amplify the genome DNA of three groups Pteria penguin materials（Guangxi Weizhou island,Guangdong Zhanjiang and Hainan Lingshui）,so the new reaction system was feasible method to conduct the AFLP analysis of Pteria penguin.
出处
《西南农业学报》
CSCD
北大核心
2011年第1期340-346,共7页
Southwest China Journal of Agricultural Sciences
基金
国家科技支撑计划(2007BAD29B03-5)
