摘要
从大肠杆菌感染的家蚕蛹提取 Ｒ Ｎ Ａ， 用 Ｒ Ｔ Ｐ Ｃ Ｒ 方法扩增未知抗菌肽基因片段， 经过克隆测序， 获得了蚕抗菌肽 Ａ 基因的部分片段１６４ ｂｐ ， 为制备蚕抗菌肽 Ａ 基因探针，
Total RNA was prepared from fat body of the silkworm, Bombyx mori , 9 hours after injected by E.coli K12D31. Single strand cDNA was synthesized by reverse transcription (RT). Partial fragments of cecropin A cDNA were obtained, cloned and sequenced, by PCR technique with a pair of degenerate primers designed according to the amino acid sequence of CM4 in Bombyx mori and cecropin A in Hyalophora . These laid a foundation for further research on preparation of the probes of cecropin A for screening the silkworm cDNA library.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第4期399-402,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
