摘要
目的:探索调节性T细胞(regulatoryT cells,Tregs)在慢性荨麻疹(CU)发病中的作用。方法:采用流式细胞术分别检测CU患者和健康人外周血CD3^+、CD4^+、CD8^+、CD4^+CD25^+T细胞的表达水平。结果:与健康对照组比较,CU患者外周血CD3^+T细胞比例无明显变化,CD4^+T细胞增高(P<0.05),CD4^+CD25^+T细胞表达水平显著增高(P<0.01),CD8^+T细胞数量降低(P<0.05),CD4^+/CD8^+比值明显升高(P<0.05)。自身血清皮肤试验(ASST)阳性组与阴性组的CU患者CD4^+CD25^+Tregs细胞的数量无统计学差异。结论:CU患者外周血存在Treg细胞及其他T淋巴细胞亚群的数量异常和分化失衡,这一异常在CU发病中的作用值得进一步研究。
Objective： To investigate the expression of peripheral regulatory T cells （Tregs） in the chronic urticaria （CU）. Methods： Peripheral blood samples were obtained from CU patients and healthy controls in this study. The frequency of CD3^＋, CD4^＋, CD8^＋ and CD4^＋ CD25^＋ in peripheral blood samples were detected by flow cytometry. Results： The increased frequency expression of CD4^＋ and CD4^＋CD25^＋ T cells was observed in CU group （n = 23） compared to control subjects （n = 19）, the ratio of CD4^＋/CD8^＋ increased significantly （P 〈 0.05）. The frequency of CD8^＋ T cells in patients of CU decreased significantly （P 〈 0.05）. No significant difference was detected in the expression levels of CD3^＋ T cells. There was no difference between autologous serum skin test（ASST） positive group and negative one in the proportion of Tregs in peripheral blood samples. Conclusion： The results demonstrate that the differentiation unbalance in T lymphocytes subgroups and the abnormal expression of regulatory T cells in peripheral blood may be involved in the pathogenesis of chronic urticaria.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2011年第3期136-138,共3页
Journal of Clinical Dermatology
关键词
荨麻疹
慢性
T淋巴细胞亚群
调节性T细胞
流式细胞术
chronic urticaria
T lymphocytes subgroups
regulatory T cells
flow cytometry
autologous serum skin test
