摘要
利用不同浓度Ｃｄ２＋、Ｈｇ２＋处理菱幼苗，研究重金属离子对菱生长、超氧化物歧化酶（ＳＯＤ）、过氧化物酶（ＰＯＤ）活性的影响，比较Ｃｄ２＋、Ｈｇ２＋对同一植物的毒性差异。Ｃｄ２＋、Ｈｇ２＋各处理浓度均抑制菱幼苗生长，使叶绿素含量下降，但Ｃｄ２＋的抑制作用比Ｈｇ２＋的作用明显。Ｃｄ２＋、Ｈｇ２＋对ＳＯＤ、ＰＯＤ活性有不同的影响效果：Ｃｄ２＋处理最初诱导ＳＯＤ、ＰＯＤ活性升高，但随浓度加大时间延长酶活性急剧下降；Ｈｇ２＋处理的酶活性变化相对平缓，其中５μｍｏｌ／Ｌ和１０μｍｏｌ／ＬＨｇ２＋处理的ＰＯＤ活性持续上升。实验结果表明，在相同处理时间和浓度条件下，Ｃｄ２＋比Ｈｇ２＋对菱的毒性要大。依据实验结果。
Treating the Seedling of Trapa bispinosa using different concentrations of Cd 2+ and Hg 2+ ,the effects of Cd 2+ and Hg 2+ on growth and activity of superoxide dismutase (SOD) and peroxidase (POD) in the species were studied.All treatments with different concentrations Cd 2+ 、Hg 2+ can inhibit growth and reduce chlorophyll content of T.bispinosa seedlings,but the inhibition of Cd 2+ are more significant than that of Hg 2+ .Effects of Cd 2+ and Hg 2+ on activity of SOD and POD are different:treatment with Cd 2+ can initially induce the activity of SOD and POD to rise,but with extending time and increasing concentration,the activity declines sharply;while activity of SOD and POD treated with Hg 2+ relatively changes smoothly,in which activity of POD treated with 5 μmol/L and 10 μmol/L Hg 2+ rises continually.Experimental results indicate that toxicity of Cd 2+ on T.bispinosa seedlings is heavier than that of Hg 2+ under the same treating time and concentration.According to the results,authors try to inquire into the toxicity mechanism of heavy metal on plants.
出处
《武汉植物学研究》
CSCD
1999年第3期206-210,共5页
Journal of Wuhan Botanical Research
基金
国家自然科学基金
江苏省自然科学基金
