摘要
目的探讨塞来昔布和洛伐他汀对前列腺癌细胞小窝蛋白-1表达及下游信号分子的影响。方法体外培养前列腺癌细胞PC-3,MTT检测塞来昔布和洛伐他汀对其增殖的影响。用IC50浓度的塞来昔布和洛伐他汀与PC-3孵育,Western blot检测小窝蛋白-1的表达以及Akt、ERK1/2和STAT3的磷酸化水平。结果 PC-3细胞经洛伐他汀和塞来昔布处理24h后I,C50值分别为25μM和50μM;塞来昔布或洛伐他汀单独处理PC-3细胞24h,能轻微下调小窝蛋白-1的表达,当两者联合使用时,能使小窝蛋白-1水平进一步降低;洛伐他汀、塞来昔布能抑制EGF诱导的Akt、ERK1/2和STAT3磷酸化,当两者共同处理PC-3细胞时,能进一步抑制其磷酸化水平。结论塞来昔布能协同洛伐他汀下调前列腺癌细胞小窝蛋白-1的表达,从而可能影响下游信号分子的激活。
Objective To explore the effect of celccoxib and lovastatin on the expression of prostate cancer cells caveolin-1 and activation of the downstream signaling molecules. Methods Prostate cancer cell line PC-3 cells were cultured in vitro, MIT test was used to detect the IC50 of celecoxib and lovastatin on cell proliferation,then cells were incubated with IC50 concentrations of celeeoxib and lovastatin,and Western blot was used to detect the expression of caveolin-1 and the phosphorylation levels of Akt,ERK1 / 2 and STAT3. Results PC-3 ceils after lovastatin and celecoxib 24h treatment,the IC50 values were 25 μM and 50p, M respectively：PC-3 cells treated with lovastatin or celecoxib separately 24h,slight down-regulated caveolin-1 expression. When the two were jointly used, they could further reduce the expression level of caveolin-1. Lovastatin and celecoxib could also inhibited EGF-induced Akt,ERK1/2 and STAT3 phosphorylation. When the two work together on PC-3 cells,they could further inhibit their phosphorylation. Conehlsion Celecoxib and lovastatin synergistically down-regulate the expression of caveolin-1,and then may affect the activation of downstream signaling molecules in prostate cancer cells.
出处
《中国现代医生》
2011年第5期14-16,共3页
China Modern Doctor
