摘要
我国乙型肝炎患者乙肝病毒前核心基因的分析卫清，张菁，闻玉梅（上海医科大学医学分子病毒室上海２０００２３）关键词乙型肝炎病毒ｅ抗原，乙型肝炎病毒前核心基因，核苷酸序列分析乙型肝炎病毒（ＨＢＶ）的核衣壳是由核心基因（Ｃ）编码，由１８３个氨基酸组成。Ｃ基因...
ariations in the sequences of the Pre-core region of hepatitis B virus(HBV)genomes iso-lated from sera of chinese chronic hepatitis B patients were studied.The entire Pre -core re-gions of HBV DNA were amplified by polymerase chain reaction (PCR) from 8 anti-HBepositive and 2 HBeAg positive chronic carriers. The amplified fragment were either cloned intoM13mp19 bacteriophage vector and at least three clones from each sample were sequenced orsequenced by PCR-direct sequencing method.By comparing the Pre-core sequences to that ofa previously cloned chinese prototype HBV DNA sequece (pADR-I)10 types of Pre-coremutations were found Six out of 10 types have a TAG stop codon mutation at codon 28 ofthe pre-core region.Two types show mutation from G to A at nucleotide 1901.One typehas a mutation which eliminates the translational initiation codon of the Pre-core gene andanother one type has a point mutation of nucleotide 1824 from T to C which does not leadto amino acid alteration.Our data demonstrate that the most prevalent type of mutation inthe HBV Pre-core region among chinese patients is the generation of a translational stopcodon.
出处
《病毒学报》
CAS
CSCD
北大核心
1994年第1期63-67,共5页
Chinese Journal of Virology
基金
国家自然科学基金
关键词
乙型肝炎
乙型肝炎病毒
前核心基因
Hepatitis B virus e-antigen,Hepatitis B virus Pre-core gene,Nucleotide sequencing Shanghai Dahua Hospital To whom correspondence and reprint requests should be sent
