摘要
以白水生姜茎尖为材料进行组培脱毒及离体快繁体系建立研究,结果表明,姜块催芽并在40～45℃光照箱中热处理30 d,再结合微茎尖(0.1～0.3 mm)分生组织培养,能有效脱除白水生姜烟草花叶病毒(TMV)和黄瓜花叶病毒(CMV)。以MS为基本培养基,添加6-BA 2.0 mg/L、NAA 0.1 mg/L有利于茎尖诱导分化;最适试管苗增殖培养基为MS+6-BA 3.0 mg/L+NAA 0.03 mg/L,增殖系数达7倍,丛芽叶绿、壮,长势好;生根培养基以MS+NAA 0.5 mg/L+IBA0.1 mg/L为宜,生根率达100%,纤维根多。
In order to investigate in vitro propagation of ginger detoxification and system building method,useing Baishuiginger stem tissue as material was studied.The results showed that tuber germination and light in the 40-45℃ heat treatment box 30 d,combined with micro-tip（0.1-0.3 mm） meristem culture,could effectively remove Baishui ginger TMV,CMV virus.By MS as basic medium supplemented with 6-BA 2.0 mg/L,NAA 0.1 mg/L help tip induced differentiation;to MS＋6-BA 3.0 mg/L＋NAA 0.03 mg/L as the optimal vitro proliferation medium,up to 7 times the multiplication coefficient,cluster shoots green,strong,growing well;to MS＋ NAA 0.1mg/L＋ IBA 0.5 mg/L for rooting,rooting rate reached 100%,many fibrous roots.
出处
《长江蔬菜》
2011年第4期17-20,共4页
Journal of Changjiang Vegetables
基金
安顺市科技攻关项目(20081003号)
贵州省科技厅农业攻关项目(黔科合NY字[2009]3027)
关键词
白水生姜
组培脱毒
离体快繁
White ginger
Detoxification
Vitro propagation
