摘要
目的:观察加味四君子汤对阿司匹林致大鼠胃黏膜损伤的修复作用。方法:50只雄性Wistar大鼠,10只作为正常对照,余40只灌服阿司匹林300mg/kg,5h后分为4组:造模验证组(以裸眼可见胃黏膜出现溃疡长径≥10mm或黏膜出血为造模成功)、自然恢复[生理盐水15mL/(kg.d)灌胃6d]组、加味四君子汤[15mL/(kg.d)灌胃6d]组和思密达[0.6g/(kg.d)灌胃5d]组。给药完成后的第1天,摘眼球取血标本测定血清IL-1β和TNF-α,脱臼处死后取胃黏液测定一氧化氮合酶(NOS)。结果:5组血清IL-1β和TNF-α以及胃黏液中NOS水平比较,差异均有统计学意义(F=8.341、18.232和14.562,P均<0.05)。与正常对照组比较,造模验证和自然恢复组IL-1β、TNF-α和NOS水平升高(P<0.05);加味四君子汤和思密达组则无明显变化(P>0.05)。结论:加味四君子汤对阿司匹林造成的胃黏膜损伤有修复作用。
Aim：To observe the effects of additional sijunzi decoction on damage of gastric mucosa induced by aspirin in rats.Methods：A total of 50 Wistar male rats were randomly allocated into 5 groups with 10 in each group.The control group did not receive aspirin.The other 4 groups were fed with aspirin at 300 mg/kg,5 hours later,modeling validation group（gastric ulcer long diameter ≥10 mm or mucosal haemorrhage for the successful modeling）,natural recovery group [saline 15 mL/（kg·d）,6 d],additional sijunzi decoction group [15 mL/（kg·d）,6 d],and smecta group [0.6 g/（kg·d）,5 d]were respectively treated as above.The blood samples were taken by removing the eyes to detect the levels of IL-1β and TNF-α,and the NOS content in gastric mucosa was also detected using ELISA.Results：Serum IL-1β and TNF-α,NOS contents in gastric mucosa among the 5 groups had significant differences（F=8.341,18.232,and 14.562,P〈0.05）.Compared with those of the control group,the IL-1β,TNF-α and NOS contents were higher in modeling validation and natural recovery groups（P〈0.05）,but had no differences in additional sijunzi decoction and smecta groups（P〈0.05）.Conclusion：Additional sijunzi decoction has a repairing effect on gastric mucosa damage induced by aspirin.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2011年第1期77-79,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省省属科研机构研究开发专项资金资助项目0641130102
关键词
胃黏膜损伤
加味四君子汤
阿司匹林
大鼠
damage of gastric mucosa
additional sijunzi decoction
aspirin
rat
