摘要
目的：用组织工程方法再造软骨。方法：分离、培养、扩传兔软骨细胞，接种于牛跟腱Ⅰ型胶原中形成软骨细胞／胶原凝胶复合物，倒置显微镜下观察细胞在凝胶中的生长情况。将复合物植入动物体内１、２ 月，观察软骨的再造情况。结果：软骨细胞在胶原凝胶中生长良好，植入体内１个月，形成软骨样组织，２ 个月时再造出成熟的软骨。结论：采用体外培养软骨细胞，接种于胶原凝胶中，是再造软骨组织的有效方法。
砄bjective: To reproduce cartilage with tissue engineering technique. Method: Rabbit ear chondrocytes were isolated and cultured in vitro and seeded into bovine type Ⅰ collagen to form chondrocyte/collagen gel composition. Chondrocytes growth in the gel and reproduction of cartilage by the composition were observed with in vitro and in vivo experiments. Results: Chondrocytes grew well in collagen gel in vitro . One month after implantation of the composition, cartilage was observed in the specimen, mature cartilage was observed in two months. Conclusion: Type I collagen/chondrocyte composition implantation is effective for tissue engineering of cartilage.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
1999年第5期381-382,共2页
Journal of Practical Stomatology
基金
国家自然科学基金
军队医药卫生科研基金
