摘要
目的检测特发性血小板减少性紫癜（ITP）患者血清白细胞介素11（IL-11）水平、淋巴，细胞亚群及NK细胞的变化，探讨相关因素在ITP发病中的作用。方法应用酶联免疫吸附法（ELISA）、流式细胞术分别检测50例ITP（ITP组）和30例健康体检者（对照组）血清IL-11水平、淋巴细胞亚群及NK细胞的变化。结果ITP组的血小板计数[（30．21±19．40）×109／L]明显低于对照组[（207．21±31．55）×10^9／L]（P〈0．05），ITP组患者血清IL．11水平[（255．72±163．43）ng／L]明显高于对照组[（40．60±5．57）ng／L]（P〈0．05），相关分析表明ITP患者血清IL-11水平与血小板计数呈负相关（r=-0．557，P〈0．05）；ITP组患者CD3＋、CO；T淋巴细胞百分比及CD4^＋／CD8^＋明显低于对照组（P〈0．05），CD8^＋T淋巴细胞百分比明显高于对照组（P〈0．05）；CD3^-CD16-56）^＋NK细胞百分比明显低于对照组（P〈0．05）。结论IL-11水平、淋巴细胞亚群及NK细胞变化与ITP的发病密切相关，且IL-11水平与血小板计数可能存在负反馈调节作用。
Objective To detect the serum level of interleukin （IL）-11, lymphocyte subsets and NK cells in patients with idiopathic thrombocytopenic purpura （iTP）, and explore the related factors in the pathogenesis of ITP. Methods The serum level of IL- 11, lymphocyte subsets and NK cells were detected by double antibody sandwich enzyme linked immunosorbent assay （ELISA） and flow cytometry in 50 ITP patients （ITP group） and 30 controls（control group）. Results The platelet in ITP group [（30.21 ± 19.40） × 10^9/L] was lower than that in control group [（207.21 ± 31.55） × 10^9/L] obviously （P〈0.05）; the serum level of IL-11 in ITP group [（255.72 ± 163.43） ng/L] was significantly higher than that in control group [ （40.60 ± 5.57） ng/L ] （P 〈 0.05 ）. The correlation analysis indicated that the blood serum levels of IL-11 had negative relationship with the platelet （r = -0.557,P 〈 0.05 ）. The percentage of CD3^＋ and CD4^＋T lymphocyte percentage, CD4^＋/CD8^＋ in ITP .group were lower and the percentage of CDs ± T lymphocyte was higher than those in control group obviously （P 〈 0.05 ）. The percentage of CD3^- CD3^- 16＋56） ^＋NK cell in ITP group was lower than that in control group （P 〈 0.05）. Conclusion IL-11, lymphocyte subgroup and NK cell change correlate with ITP morbidity closely, and the IL-11 level and the platelet possibly have the negative feedback control action.
出处
《中国医师进修杂志》
2011年第4期27-29,共3页
Chinese Journal of Postgraduates of Medicine
