摘要
目的 探讨体外膀胱灌注噻替哌和丝裂霉素Ｃ 药物浓度和作用时间对膀胱癌细胞杀伤作用的影响。 方法 用不同浓度的抗肿瘤药物与人膀胱移行细胞癌细胞系ＢＩＵ８７ 共同培养至一定时间，采用ＭＴＴ 比色法测定细胞存活率。 结果 细胞存活率与二药的药物浓度和作用时间均呈明显的线性相关（ Ｐ＜ ０ ．０１） ，而且主要与浓度有关；当超过一定作用时间后，存活率仅与药物浓度呈负相关（ Ｐ＜ ０ ．０１） 。 结论 只有保证有效的作用时间，尽量增加药物剂量和有效浓度，才能显著提高膀胱腔内化疗效果，减少复发。
Objective To study the cytotoxic effect of thiotepa (THT) and mitomycin C (MMC) on human bladder cancer cell line in vitro. Methods Human bladder transitional cell carcinoma cell line BIU 87 was cultured for varying period with varying concentrations of the antineoplastic agents added.MTT colorimetric assay was used to determine the cell survival rate. Results Drug concentration and exposure period were significantly correlated with the cell survival rate ( P <0.01),drug concentration being the predominant.When the effective exposure period for the drugs was ensured and accessed the cell survival rate was only negatively correlated with drug concentration ( P <0.01). Conclusions When the effective exposure period is guarranted,increase of drug dose and the effective concentration would significantly enhance the therapeutic effect of the intravesical chemotherapy,thus reducing the recuurence rate of bladder cancer after surgical management.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1999年第10期616-618,共3页
Chinese Journal of Urology
