摘要
目的研究抑癌基因PTEN转染对人膀胱癌细胞系BIU-87化疗敏感性的影响。方法将携有PTEN基因的重组真核表达质粒pBp-PTEN体外转染BIU-87细胞(pBp-PTEN-BIU87),筛选稳定转染的细胞并扩增培养,以转染了空质粒pBp的BIU-87细胞(pBp-BIU87)和正常BIU-87细胞为对照,用RT-PCR检测PTEN的表达情况,应用MTT分别研究转染前、后膀胱癌细胞对不同浓度抗癌药物丝裂霉素MMC(1、10和100g/mL)、康朴赛星(0.25、2.5和25g/mL)和吡柔比星(2、20和200g/mL)的抑制率和敏感性的变化。结果 RT-PCR证实PTEN在转染的BIU-87细胞中能稳定表达。PTEN转染后,BIU-87细胞对MMC的药物抑制率和敏感性明显提高,对吡柔比星和康朴赛星的药物抑制率和敏感性无明显变化。结论 PTEN基因转染能提高人膀胱癌细胞系BIU-87对某些抗癌药物的敏感性,作用的强弱取决于药物的作用机制。
Objective To study the effects of antioncogene PTEN transfection on chemosensitivity in human bladder cancer cell line BIU-87. Methods An eukaryotic expression plasmid containing PTEN,pBp-PTEN was transfected into BIU-87 （pBp-PTEN-BIU87）,and positive cell clones were selected and amplified. With empty plasmid transfected BIU-87 （pBp-BIU87） and normal BIU-87 as the control groups,expression of PTEN was detected by RT-PCR. Chemosensitvity of BIU-87 to mitomycin （MMC,1 g/mL,10 g/mL or 100 g/mL）,hydro-xycamptothecin for injection （0.25 g/mL,2.5 g/mL or 25 g/mL） and pirarubicin （2 g/mL,20 g/mL or 200 g/mL） before and after transfection were measured by MTT. Drug concentration was calculated according to 0.l,1.0 and 10 times of clinical plasm peak concentration （PPC）. Drug inhibit rate （IR） = （Absorbency of control-Ab-sorbency of test）/Absorbency of control×100%. IR 25%,it was not sensitive; 25%≤IR50%,sensitive; 50≤ IR75%,median sensitive; IR≥75%,highly sensitive. Results After transfection of the gene,the chemosensitivity of BIU-87 to mitomycin was increased significantly,but there were no changes in chemosensitivity of BIU-87 to hydroxycamptothecin and pirarubicin. Conclusions The data indicated that PTEN might increase the chemosensitivity to some chemotherapy drugs which depend on the different mechanisms of drugs.
出处
《现代实用医学》
2010年第4期369-371,F0003,共4页
Modern Practical Medicine
基金
宁波市医学科技计划项目
编号:2006026
