摘要
为实现实验教学与科研工作的紧密结合,该教学设计采用激光扫描共聚焦显微镜在细胞原位检测核酸和绿色荧光蛋白,分析融合蛋白的亚细胞定位。通过2个经典的检测方案,使学生更好地理解和掌握激光扫描共聚焦显微镜的检测原理、操作步骤及结果分析方法。
The confocal laser scanning microscope is an important technique in biology.To combine the experimental teaching with scientific research tightly,an experiment was designed to detect nucleic acid and protein in situ by the confocal laser scanning microscope.Two classical detection protocols were employed,which provided the students with a better understanding and mastering of the detection principle,the operation procedure and the analysis method.
出处
《实验技术与管理》
CAS
北大核心
2011年第2期58-60,共3页
Experimental Technology and Management
基金
北京市教委科研项目(KM200810005015)
北京市自然科学基金(5072003)
北京工业大学研究生课程建设项目(CR2008-B-034)
北京工业大学2009年校青年基金
关键词
激光扫描共聚焦显微镜
绿色荧光蛋白
碘化丙啶
confocal laser scanning microscope
green fluorescent protein
propidium iodide
