摘要
目的探讨激光共聚焦扫描显微镜(LCSM)技术在动态测定神经元细胞内钙离子浓度中的应用。方法采用原代培养大鼠皮层神经元,用LCSM测定给KCl前后细胞内钙离子浓度的动态变化。结果培养神经元状态良好,用LCSM准确、稳定、可靠地测出细胞内钙离子浓度的动态变化。结论LCSM在动态测定神经元细胞内钙离子浓度中具有明显优势。
Objective To document the promises of laser confocal scanning microscopy （LCSM） in dynamic study of intracellular calcium concentration （[Ca^2＋]i） in neurons subjected to different drug administration. Methods LCSM was used to investigate the dynamic change of [Ca^2＋]i in primary cultured cortical neurons before and after KCl treatment. Results The cultured neurons showed good morphology and growth status. Accurate, stable and reliable measurements of dynamic [Ca^2＋]i were performed using LCSM. Conclusion LCSM technique may show promises for measurement of dynamic change in [Ca^2＋]i.
出处
《中国药物与临床》
CAS
2008年第7期530-532,F0003,共4页
Chinese Remedies & Clinics
基金
国家自然科学基金科学部主任基金资助项目(30740095)
教育部高等学校博士学科点专项科研基金资助项目(20060114004)
山西省自然科学基金资助项目(200601105)
山西省重点实验室开放基金资助项目(200603012)
关键词
显微镜检查
共焦
细胞内钙离子浓度
皮层神经元
Microscopy, confocal
Intracellular calcium concentration
Cortical neurons
