摘要
采用响应面分析法对杆菌肽产生菌的发酵培养基进行优化。采用Plackett-Burman实验筛选出影响杆菌肽产量的主要因素为豆粕、Na2S2O3、生物氮素,利用最陡爬坡路径逼近响应区域,应用Box-Behnken设计和响应面分析优化得到最佳发酵培养基,发酵单位较优化前提高了36.9%。
The fermentation materials of bacitracin producing strain were optimized by the response surface methodology.First,Plackett-Burman design was adopted to screen out the important factors affecting the output of bacitracin：soybean meal,Na2S2O3,biological nitrogen.Then the path of the steepest ascent was utilized to approach the optimal region.At last,the optimized medium was obtained by Box-Behnken design and response surface methodology.Under the condition,the fermentation-titer was increased by about 36.9% compared with the previous condition.
出处
《河北化工》
2011年第2期17-19,55,共4页
Hebei Chemical Industry
关键词
杆菌肽
响应面
优化
培养基
bacitracin
response surface methods
optimization
medium
