摘要
目的 观察长期高蛋白、低碳水化合物饮食对营养性肥胖大鼠体重和胃肠激素表达的影响. 方法 利用高脂饲料建立营养性肥胖大鼠模型24只,用随机数字表法按体重分层随机分为高蛋白饲养组（HP组,36.7%蛋白质）、普通饲养组（NC组,22.4%蛋白质）,每组12只.正常等热量饲养24周后,测定体重、内脏脂肪及空腹血浆胃促生长素、胰高血糖素样肽1（GLP-1）,免疫组织化学检测胃底胃促生长素、回肠GLP-1蛋白表达水平. 结果 24周后,NC、HP组体重分别为（490.92±39.47）、（545.55±31.08）g（t=-3.664,P＜0.01）;内脏脂肪分别为（22.42±7.04）、（32.33±9.27）g（t=-2.503,P＜0.05）;空腹血浆胃促生长素分别为（2.36±0.82）、（1.95±0.64）ng/ml（t=1.337,P＞0.05）,并与体重（r=-0.370,t=-1.899,P＜0.05）、内脏脂肪量（r=-0.454,t=-2.52,P＜0.01）呈负相关;空腹血浆GLP-1分别为（0.52±0.13）、（0.71±0.19）μg/L（t=-2.758,P＜0.05）;胃底胃促生长素表达分别为25 473±8701、10 526±6194（t=2.501,P＜0.05）;回肠GLP-1蛋白表达分别为27 431±5813、36 601±5083（t=-1.833,P=0.081）. 结论 长期高蛋白、低碳水化合物饮食可引起营养性肥胖大鼠体重、体脂下降,胃促生长素表达升高,GLP-1下降.
Objective To investigate the effects of long-term high-protein,low-carbohydrate diet on body weight and the expression of gastrointestinal hormones in diet-induced obesity rats. Methods Twentyfour diet-induced obesity rat models were established by feeding fat-enriched diet,then were randomly divided into two groups by stratified sampling method by weight：the high-protein diet group （HP,36.7% of energy from protein）,and the normal chow group （NC,22.4% of energy from protein）,12 rats in each group.The total calorie intake of each rat per day was similar and was maintained for 24 weeks,then body weight,visceral fat mass,fasting plasma ghrelin and glucagon-like peptide-1 （GLP-1） were determined,as well as protein expression of ghrelin in stomach,GLP-1 in ileum were detected by immunohistochemistry. Results After 24 weeks,body weight of HP,NC groups were （490.92±39.47）g and （545.55±31.08）g,respectively （t=-3.664,P〈0.01）;visceral fat mass were （22.42±7.04）g and （32.33±9.27） g,respectively（t=-2.503,P〈0.05）;plasma ghrelin level were （2.36±0.82） and （1.95±0.64） ng/ml,respectively（t=1.337,P〉0.05）,and plasma ghrelin level was negatively correlated to body weight （r=-0.370,t=-1.899,P〈0.05）,visceral fat mass（r=-0.454,t=-2.52,P〈0.01）;plasma GLP-1 concentration were （0.52±0.13）and（0.71±0.19）ng/ml,respectively（t=-2.758,P〈0.05）;ghrelin protein expression in stomach were 25 473±8701 and 10 526 ± 6194,respectively （t=2.501,P〈0.05）;GLP-1 prot ein expression in ileum were 27 431±5813 and 36 601±5083,respectively （t=-1.833,P=0.081）. Conclusion Long-term isocaloric high-protein,low-carbohydrate diet can reduce body weight and visceral fat,increase the expression of ghrelin,and decline GLP-1 expression in diet-induced obesity rats.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2011年第2期132-135,共4页
Chinese Journal of Preventive Medicine
基金
山东省自然科学基金（Z2006C09）
