摘要
用６ － 二甲基氨基嘌呤（６ － ＤＭＡＰ） 处理，对抑制栉孔扇贝第二极体释放产生三倍体的适宜诱导参数进行了试验研究，结合ＤＡＰＩ染色和荧光显微镜观察，分析了开始处理前和处理结束时的受精卵染色体核相组成与三倍体诱导率和幼虫成活率的关系，对三倍体处理组和对照组幼虫的生长进行了观察。结果表明，获得充分成熟的卵子、起始处理时间以第一极体占４０ ％ 、药物浓度６０ ｍｇＬ、持续处理时间１５ ｍｉｎ 及产卵至处理结束保持水温２０ ℃，三倍体诱导率可稳定在８０ ％以上，孵化率达７０ ％ 左右。
To produce tripoids, fertilized eggs of Chlamys(Azumapecten)farreri were treated with 6-dimethylaminopurine (6-DMAP) to inhibit the extrusion of the second polar body. Using dihydrochloride (DAPI) stain and fluorescence microscopic observation, the relation between chromosome karyotype and triploid induction and larval survival percentage, the effects of egg maturation and spawning temperature on the synchronous development of fertilized eggs were analyzed. It was concluded that the optimism induction parameters for farming industry are as following: completely mature eggs, initial treatment time when the first polar body occupied 40%, 60mg/L of drug concentration, 15 min of treatment duration and water temperature kept at 20℃ from spawning to the end of treatment. Under the conditions, triploids can be obtained with an induction rate higher than 80% and an hatching rate up to 70%.
出处
《水产学报》
CAS
CSCD
北大核心
1999年第3期241-247,共7页
Journal of Fisheries of China
基金
国家海洋863 计划资助
关键词
栉孔扇贝
二甲基氨基嘌呤
三倍体诱导
Chlamys farreri , 6-Dimethylaminopurine(6-DMAP), Triploid induce
