摘要
用 Ｐ Ｃ Ｒ 技术对２７４ 例无肾病并发症的Ⅱ型糖尿病（ Ｎ Ｉ Ｄ Ｄ Ｍ） 、１０２ 例Ⅱ型糖尿病肾病合并肾功能不全（ Ｒ Ｉ） 、１２４ 例Ⅱ型糖尿病肾病无肾功能不全（ Ｎ Ｒ Ｉ） 、２４２ 例正常对照组进行了血管紧张素Ⅰ转换酶（ Ａ Ｃ Ｅ） 基因插入／ 缺失多态性的检测，同时观察血清血管紧张素转换酶（ Ａ Ｃ Ｅ） 水平与 Ａ Ｃ Ｅ 基因多态性及疾病的关系。结果： Ｒ Ｉ组 Ｄ 等位基因频率０ ．５２ ， Ｄ Ｄ 基因型频率０ ．３０ ，与对照组（０ ．３８ ，０ ．１６） 比较有显著差异（ Ｐ＜ ０ ．０５ ， Ｐ＜ ０ ．００１） ； Ｄ Ｄ 基因型较 Ｉ Ｉ对 Ｒ Ｉ 的比数比为２ ．９１（９５ ％ Ｃ Ｉ为１ ．５２ ～５ ．５８ ， Ｐ＜ ０ ．０１） 。 Ｎ Ｉ Ｄ Ｄ Ｍ 组及 Ｎ Ｒ Ｉ组基因型频率分布与对照组比较无显著差异（ Ｐ＞ ０ ．０５） ；各组中 Ｄ Ｄ 基因型个体血清 Ａ Ｃ Ｅ 水平最高 Ｉ Ｉ基因型最低， Ｒ Ｉ组及 Ｎ Ｒ Ｉ组血清 Ａ Ｃ Ｅ 水平显著高于对照组（ Ｐ＜ ０ ．００１） ，基因多态性与血清 Ａ Ｃ Ｅ 水平呈相关性（ ｒ ＝ ０ ．６５ ， Ｐ＜ ０ ．０１） 。表明血清 Ａ Ｃ Ｅ 水平升高对糖尿病肾病的发生及进展有重要作用， Ａ Ｃ Ｅ
Angiotensin Ⅰ converting enzyme (ACE) genotypes of 274 cases with type Ⅱ diabetes mellitus without nephropathy (NIDDM), 102 with diabetic nephropathy complicated with renal insufficiency (RI) and 124 diabetic nephropathy without renal insufficiency (NRI), and 242 controls were determined by polymerase chain reaction (PCR) amplification of the insertion/deletion polymorphism of ACE gene. Serum ACE levels were measured with ultraviolet photometry as well. The increased frequencies of D allele (0.52) and DD genotype (0.30) were observed in 102 subjects with RI compared with 242 control subjects (0.38 and 0.16 respectively, P <0.05 and P <0.001). The odds ratio associated with DD/II genotype was 2.91 (95% CI 1.52～5.58, P <0.01) for RI. Individuals with DD genotype had the highest serum ACE in all groups, and with II genotype the lowest levels. Patients with diabetic nephropathy and RI had higher serum ACE levels compared with controls ( P <0.001). There was no difference in allele distribution between 274 NIDDM patients, 124 patients with NRI and the controls. ACE gene polymorphism is associated with serum ACE levels ( r =0.65, P <0.01). This result suggested that the raised serum ACE concentration may play a role in the initiation and development of diabetic nephropathy; deletion polymorphism of the ACE gene might be an important risk factor of progression to end stage renal failure in Chinese type Ⅱ diabetes patients.
出处
《首都医科大学学报》
CAS
1999年第3期174-177,共4页
Journal of Capital Medical University
关键词
基因多态性
糖尿病肾病
ACE
angiotensin converting enzyme
gene polymorphism
diabetic nephropathy
