摘要
目的:观察来氟米特对子宫内膜异位症大鼠模型病灶生长行为的影响,为子宫内膜异位症的免疫治疗提供依据。方法:采用自体移植法建立大鼠子宫内膜异位症模型。移植第3周治疗组给予来氟米特35 mg/kg.d,对照组给予生理盐水1 m l/kg.d,连用3周;测量用药前后病灶体积,并采用放免法检测TNF-α水平,免疫组化法检测NF-κB的表达情况。结果:治疗组病灶体积显著低于对照组(P<0.05),组织病理学显示治疗组的异位内膜生长明显受抑制或变性坏死,病灶组织血供不足;治疗组TNF-α及NF-κB表达显著低于对照组(P<0.05)。结论:来氟米特对大鼠子宫内膜异位病灶的生长有明显抑制作用,证实免疫调节治疗子宫内膜异位症的策略具有可行性。
Objective：To observe the effect of leflunomide on focal growth behaviors of rat models of endometriosis,provide a basis for immune therapy of endometriosis.Methods：Autologous transplantation was used to established rat models of endometriosis;on the third week after transplantation,the rats in treatment group were treated with leflunomide,35 mg·kg-1·d-1 for three weeks;the rats in control group were treated with normal saline,1 ml·kg-1·d-1 for three weeks;the focal volumes before and after treatment were measured,radioimmunoassay was used to detect the expression of TNF-α,and immunohistochemical method was used to detect the expression of NF-κB.Results：The focal volume of treatment group was significantly smaller than that of control group（P〈0.05）;histopathological results showed that the growth of ectopic endometrium in treatment group was inhibited or necrosis,the blood supply of focal tissues was inadequate;the expression levels of TNF-α and NF-κB in treatment group were significantly lower than those in control group（P〈0.05）.Conclusion：Leflunomide can inhibit the focal growth of endometrium in rat models,it is feasible to treat endometriosis by immune regulation.
出处
《中国妇幼保健》
CAS
北大核心
2011年第3期428-430,共3页
Maternal and Child Health Care of China
