摘要
采用甲醇为溶剂，提取冬虫夏草及其虫体、子座中的核苷类成分，用ＨＰＬＣ法测定腺苷的含量；色谱条件以μＢｏｎｄａｐａｋ Ｃ_(１８)（４．６ ｍｍ × ２５０ ｍｍ，１０μｍ）为色谱柱，０．０１ ｍｏｌ／Ｌ磷酸二氢钠-磷酸氢二钠缓冲液（ｐＨ６．５）－ 甲醇（８５： １５）为流动相，检测波长 ＵＶ２６０ ｎｍ，ｒ＝０．９９９９，线性范围 ０．２～１μｇ，回收率１０４．５％。
A method has been developed for the determination of adenosine in Cordycepes sinensis (Berk.) Sacc. by HPLC. Nucleosides in both worm body and stroma were extracted with MeOH. A μ Bon- dapak C_(18) column was used, with phosphate buffer (0. 01 mol/L of a mixture of NaH_2PO_4 and Na_2HPO_4 of pH 6. 5)-MeOH (85:15) as the mobile phase, and detected at a wavelength of 260 nm. The linear range was 0. 2-1. 0μg, r= 0. 9999.The average recovery was 104. 5 %.
出处
《中草药》
CAS
CSCD
北大核心
1999年第9期658-660,共3页
Chinese Traditional and Herbal Drugs
